Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NBC2815_RS13950 Genome accession   NZ_LT853880
Coordinates   3023581..3024618 (-) Length   345 a.a.
NCBI ID   WP_002802057.1    Uniprot ID   -
Organism   Xanthomonas fragariae strain NBC2815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3018581..3029618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBC2815_RS13920 (NBC2815_02777) ruvX 3019218..3019685 (-) 468 WP_088056710.1 Holliday junction resolvase RuvX -
  NBC2815_RS13925 (NBC2815_02778) - 3019678..3020244 (-) 567 WP_002802054.1 YqgE/AlgH family protein -
  NBC2815_RS13930 (NBC2815_02779) - 3020540..3021097 (+) 558 WP_088061738.1 DNA-3-methyladenine glycosylase I -
  NBC2815_RS13940 - 3021298..3022197 (+) 900 Protein_2704 DUF72 domain-containing protein -
  NBC2815_RS13945 (NBC2815_02782) pilU 3022344..3023474 (-) 1131 WP_002802056.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NBC2815_RS13950 (NBC2815_02783) pilT 3023581..3024618 (-) 1038 WP_002802057.1 type IV pilus twitching motility protein PilT Machinery gene
  NBC2815_RS13955 (NBC2815_02784) - 3024972..3025664 (+) 693 WP_088062595.1 YggS family pyridoxal phosphate-dependent enzyme -
  NBC2815_RS13960 (NBC2815_02785) proC 3025722..3026576 (+) 855 WP_088061740.1 pyrroline-5-carboxylate reductase -
  NBC2815_RS13965 (NBC2815_02786) - 3027139..3027564 (+) 426 WP_040761922.1 HU family DNA-binding protein -
  NBC2815_RS13970 (NBC2815_02787) - 3027671..3028312 (+) 642 Protein_2710 M23 family metallopeptidase -
  NBC2815_RS13975 (NBC2815_02788) - 3028404..3028712 (+) 309 WP_002802064.1 DUF3861 family protein -
  NBC2815_RS13980 (NBC2815_02789) - 3028825..3029421 (+) 597 WP_002802066.1 DUF1439 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38688.34 Da        Isoelectric Point: 6.6652

>NTDB_id=1146425 NBC2815_RS13950 WP_002802057.1 3023581..3024618(-) (pilT) [Xanthomonas fragariae strain NBC2815]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIQSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1146425 NBC2815_RS13950 WP_002802057.1 3023581..3024618(-) (pilT) [Xanthomonas fragariae strain NBC2815]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCGTCGGACCTGCACCTGTCTGCAGGCCTGCCGCCGAT
GATCCGAGTTGATGGCGATGTCCGCCGCATCAATATTCCGGCCCTGGATCACAAGCAGGTGCATGCGCTGGTCTACGACA
TTATGTCGGACAAGCAGCGCCGCGATTACGAAGAATTCCTCGAAGTCGACTTCTCGTTCGAGATCCAGTCGCTGGCACGC
TTCCGCGTCAATGCGTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTTGGCTGCCCACCGATTTTCCGGCAGCTGATCGATCAGCCGCAGGGCTTGATCCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCATCGCGACACGCACGGCTTCAACGA
AGCATTGCGCTCTGCATTGCGTGAAGATCCGGATATCATCCTGGTTGGCGAGTTGCGCGATCTTGAAACCATCCGCTTGG
CACTCACCGCTGCGGAAACCGGCCATCTGGTGTTCGGTACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGATGTCTTCCCTGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCCTTGCGCGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGACGCACCGCCGCCTGGGAAATCATGGTTGGCACACCGGCCATCCGTAACCTGATCC
GGGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTGGATCAGCATTTA
CAGGACCTGATCAAACGCAGCCTGATCACGCGCAACCAAGCACGCGAATACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment