Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NBC2815_RS13945 Genome accession   NZ_LT853880
Coordinates   3022344..3023474 (-) Length   376 a.a.
NCBI ID   WP_002802056.1    Uniprot ID   A0A1Y6H4M7
Organism   Xanthomonas fragariae strain NBC2815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3017344..3028474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBC2815_RS13910 (NBC2815_02775) - 3017579..3018013 (-) 435 WP_002802050.1 OsmC family protein -
  NBC2815_RS13915 (NBC2815_02776) - 3018257..3019204 (-) 948 WP_002802051.1 aspartate carbamoyltransferase catalytic subunit -
  NBC2815_RS13920 (NBC2815_02777) ruvX 3019218..3019685 (-) 468 WP_088056710.1 Holliday junction resolvase RuvX -
  NBC2815_RS13925 (NBC2815_02778) - 3019678..3020244 (-) 567 WP_002802054.1 YqgE/AlgH family protein -
  NBC2815_RS13930 (NBC2815_02779) - 3020540..3021097 (+) 558 WP_088061738.1 DNA-3-methyladenine glycosylase I -
  NBC2815_RS13940 - 3021298..3022197 (+) 900 Protein_2704 DUF72 domain-containing protein -
  NBC2815_RS13945 (NBC2815_02782) pilU 3022344..3023474 (-) 1131 WP_002802056.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NBC2815_RS13950 (NBC2815_02783) pilT 3023581..3024618 (-) 1038 WP_002802057.1 type IV pilus twitching motility protein PilT Machinery gene
  NBC2815_RS13955 (NBC2815_02784) - 3024972..3025664 (+) 693 WP_088062595.1 YggS family pyridoxal phosphate-dependent enzyme -
  NBC2815_RS13960 (NBC2815_02785) proC 3025722..3026576 (+) 855 WP_088061740.1 pyrroline-5-carboxylate reductase -
  NBC2815_RS13965 (NBC2815_02786) - 3027139..3027564 (+) 426 WP_040761922.1 HU family DNA-binding protein -
  NBC2815_RS13970 (NBC2815_02787) - 3027671..3028312 (+) 642 Protein_2710 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41975.23 Da        Isoelectric Point: 6.8287

>NTDB_id=1146424 NBC2815_RS13945 WP_002802056.1 3022344..3023474(-) (pilU) [Xanthomonas fragariae strain NBC2815]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTTLQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGHRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIVNFFPDERRNQLLMDLSLNLKGVIAQQLIPTPDGRSRRVAMEILLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1146424 NBC2815_RS13945 WP_002802056.1 3022344..3023474(-) (pilU) [Xanthomonas fragariae strain NBC2815]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTTATTACTTCGGGAAT
GCCGCCGGCAATCAAGGTTAATGGGAAGATCAGTCCGATCACCCAGACCCCGTTGACCGCGCAGCAGAGCCGCGATCTTG
TGTTGAATGTGATGACCACATTGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATTGGCGTGTCAGGG
GTGGGGCGCTTTCGTGTGAGCTGTTTTTATCAGCGCAACCAGGTCGGCATGGTGCTGCGGCGGATTGAAACGCGCATTCC
CACCGTCGACGAATTGAGTCTGCCGCCGGTGATCAAGACGCTGGCAATGACCAAGCGCGGCATCATCATTTTTGTCGGTG
CCACCGGTACCGGTAAGTCGACCTCGCTTGCAGCGATGATCGGCCACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGGGAAGTCGGAATCGATACCGATAG
CTGGGAAAATGCGCTGAAAAATACCCTGCGCCAGGCACCGGATGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATTGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACGTTGCATGCCAATAACGCCAACCAGGCGATG
GACCGCATCGTCAACTTCTTCCCGGACGAGCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAATCTCAAGGGCGTGAT
CGCGCAGCAGTTGATTCCGACCCCGGATGGACGCAGCCGTCGCGTGGCGATGGAAATCCTGCTGGGCACGCCGTTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACAAACCTGGGCATGCGCACCTTC
GATCAGAGCTTGTTCGAGCTCTACCAGGCTGGTGAAATCAGTTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGTGGTGATGCCAAGACGCTGTCGCAGGGCCTGGACGGCGTGGAAATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(83-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6H4M7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

64.986

94.947

0.617
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.577

94.947

0.404
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362

Multiple sequence alignment