Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DABAL43B_RS01735 Genome accession   NZ_LT799838
Coordinates   394575..395630 (-) Length   351 a.a.
NCBI ID   WP_079690794.1    Uniprot ID   -
Organism   Psychrobacter sp. DAB_AL43B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389575..400630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DABAL43B_RS01710 (DABAL43B_0340) - 390177..391319 (+) 1143 WP_079690790.1 hypothetical protein -
  DABAL43B_RS01715 (DABAL43B_0341) - 391307..391750 (+) 444 WP_079692998.1 RnfH family protein -
  DABAL43B_RS01720 (DABAL43B_0342) - 391776..392192 (-) 417 WP_079690791.1 outer membrane protein assembly factor BamE -
  DABAL43B_RS01725 (DABAL43B_0343) fur 392517..392951 (+) 435 WP_079690792.1 ferric iron uptake transcriptional regulator -
  DABAL43B_RS01730 (DABAL43B_0344) pilU 393123..394244 (-) 1122 WP_079690793.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DABAL43B_RS01735 (DABAL43B_0345) pilT 394575..395630 (-) 1056 WP_079690794.1 type IV pilus twitching motility protein PilT Machinery gene
  DABAL43B_RS01740 (DABAL43B_0346) - 395985..396749 (+) 765 WP_079690795.1 YggS family pyridoxal phosphate-dependent enzyme -
  DABAL43B_RS01745 (DABAL43B_0347) - 396916..398193 (-) 1278 WP_079690796.1 hemolysin family protein -
  DABAL43B_RS01750 (DABAL43B_0348) - 398446..400149 (-) 1704 WP_079690797.1 NAD+ synthase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39196.03 Da        Isoelectric Point: 6.6711

>NTDB_id=1146233 DABAL43B_RS01735 WP_079690794.1 394575..395630(-) (pilT) [Psychrobacter sp. DAB_AL43B]
MAEKNNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPEMTHQVVHQLIYDIMNDRQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFDAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMI
TLDQTLKNLVSKGTISKDVARPYAKQPEAFI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1146233 DABAL43B_RS01735 WP_079690794.1 394575..395630(-) (pilT) [Psychrobacter sp. DAB_AL43B]
ATGGCTGAAAAAAACAATTTAAGCATCGAAGATTTACTACGCTTTACAGTCAAGCATAAAGCCTCAGATTTACATATTTC
AGCCGGTATGCCAGCGATGATTCGAGTCGACGGCGAGATGACCCGTATCAATATGCCAGAAATGACTCATCAAGTGGTGC
ATCAGCTTATCTATGACATCATGAATGATAGGCAGCGTGCCGACTTTGAAGAATTCTTTGAGACGGATTTCTCTTTTGAG
ATTCCTAATTTAGCGCGTTTTCGGGTAAATGCTTTTAACCAAAATCGCGGTGCGGGCGCAGTCTTTCGTACGATTCCGTC
AAAAGTTCTCACCATGGAAGACTTAGGAATGGGTGAAGTCTTCAAACGCGTCTCCGACTTTAAGCGCGGCGTAGTATTAG
TGACTGGTCCAACCGGCTCAGGGAAATCTACAACGCTGGCGGCCATGATTGATTATATCAATGAAACCCGTAAAGAGCAT
ATTTTGACCATTGAAGACCCGATTGAATTTGTTCACGAGTCTAAAAAGAGCTTGATCAACCAGCGTGAAGTACACCGTGA
TACGTTAGGTTTCGATGCAGCACTGCGCTCAGCTTTACGTGAGGATCCCGATGTTATTCTAGTTGGTGAGCTACGTGACC
TTGAGACCATACGCTTGGCCCTCACCGCAGCTGAAACTGGTCACTTAGTCTTTGGGACTTTGCACACCAGCTCCGCAGCA
AAAACCATTGACCGTGTCATTGACGTATTCCCTGCCGCTGAAAAAGATATGATTCGTGCGATGCTATCGGAGTCGCTACA
AGCAGTGATATCCCAGACGCTGTTACGTCGTCAAACTGGTGGACGAATTGCCGCGCATGAGATTATGCTGGGTACGCCCG
CTATTCGTAACTTGATACGTGAAAACAAAATTGCCCAAATGTACTCTTCTATCCAAACGGGTGCAGGTGACGGTATGATT
ACGCTCGATCAAACGCTTAAAAACCTAGTATCAAAAGGCACGATTAGCAAAGACGTCGCACGACCTTATGCTAAGCAGCC
TGAGGCCTTTATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.551

98.291

0.772
  pilT Acinetobacter baumannii strain A118

78.261

98.291

0.769
  pilT Acinetobacter baumannii D1279779

78.261

98.291

0.769
  pilT Pseudomonas stutzeri DSM 10701

77.616

98.006

0.761
  pilT Pseudomonas aeruginosa PAK

77.616

98.006

0.761
  pilT Acinetobacter baylyi ADP1

76.163

98.006

0.746
  pilT Legionella pneumophila strain Lp02

72.384

98.006

0.709
  pilT Legionella pneumophila strain ERS1305867

72.384

98.006

0.709
  pilT Neisseria meningitidis 8013

64.638

98.291

0.635
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

93.732

0.632
  pilT Vibrio cholerae strain A1552

67.477

93.732

0.632
  pilT Neisseria gonorrhoeae MS11

64.058

98.291

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.009

98.86

0.504
  pilU Acinetobacter baylyi ADP1

41.108

97.721

0.402
  pilU Vibrio cholerae strain A1552

42.724

92.023

0.393
  pilU Pseudomonas stutzeri DSM 10701

40.413

96.581

0.39

Multiple sequence alignment