Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DABAL43B_RS01730 Genome accession   NZ_LT799838
Coordinates   393123..394244 (-) Length   373 a.a.
NCBI ID   WP_079690793.1    Uniprot ID   A0A229GJH9
Organism   Psychrobacter sp. DAB_AL43B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388123..399244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DABAL43B_RS01700 (DABAL43B_0338) - 388597..388932 (+) 336 WP_079690789.1 c-type cytochrome -
  DABAL43B_RS01705 (DABAL43B_0339) - 389257..389925 (+) 669 WP_079692997.1 c-type cytochrome -
  DABAL43B_RS01710 (DABAL43B_0340) - 390177..391319 (+) 1143 WP_079690790.1 hypothetical protein -
  DABAL43B_RS01715 (DABAL43B_0341) - 391307..391750 (+) 444 WP_079692998.1 RnfH family protein -
  DABAL43B_RS01720 (DABAL43B_0342) - 391776..392192 (-) 417 WP_079690791.1 outer membrane protein assembly factor BamE -
  DABAL43B_RS01725 (DABAL43B_0343) fur 392517..392951 (+) 435 WP_079690792.1 ferric iron uptake transcriptional regulator -
  DABAL43B_RS01730 (DABAL43B_0344) pilU 393123..394244 (-) 1122 WP_079690793.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DABAL43B_RS01735 (DABAL43B_0345) pilT 394575..395630 (-) 1056 WP_079690794.1 type IV pilus twitching motility protein PilT Machinery gene
  DABAL43B_RS01740 (DABAL43B_0346) - 395985..396749 (+) 765 WP_079690795.1 YggS family pyridoxal phosphate-dependent enzyme -
  DABAL43B_RS01745 (DABAL43B_0347) - 396916..398193 (-) 1278 WP_079690796.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41656.93 Da        Isoelectric Point: 6.9357

>NTDB_id=1146232 DABAL43B_RS01730 WP_079690793.1 393123..394244(-) (pilU) [Psychrobacter sp. DAB_AL43B]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTPSQQQEFKETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNLEVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEIHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGNANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1146232 DABAL43B_RS01730 WP_079690793.1 393123..394244(-) (pilU) [Psychrobacter sp. DAB_AL43B]
ATGGACATTGATAAACTTTTACAATTGATGATCAATAAAAATGGCTCCGACCTTTTTATTACTGCGGATGTCGCGCCTTC
CATGAAAATAAATGGAAAAATTCTGCCCGTTGGTAAAACGCCTTTAACGGCTGAGCAAACCATGAGGCTGGTCAAAGGTG
TGATGACGCCCAGCCAGCAACAAGAGTTTAAAGAGACCAACGAATGCCAGTTTGCGATTACCGATCAAGCCCAGCAAGCA
CGTTTTCGAGTCAGTGCCTTTATACAACGCGATATGGCAGGGATGATTTTACGGAAAATCGAAAATAAAATCCCGACGGT
CGAAGAGCTGCGTCTGCCGCCAGCACTAAAAGAGCTGGCAATGAAAAAACGCGGTATTATTCTATTAGTGGGTGCAACGG
GTACCGGTAAATCGACCACCCTTGCAGCGATGATTGGACACCGTAATCAAAACTCTCACGGTCACATTATTACCATCGAA
GACCCTATTGAATTTGTACATAAACATCGCGGTTGTATTATTACCCAGCGCGAAGTCGGTATCGATACTCACTCTTTTGA
AGCCGGTCTAAAAAATACGCTACGCCAAGCGCCTGATGTTATTTTGATTGGTGAGATTCGCAACCTCGAAGTTATGAGCT
ATGCAATCCAATATGCAGAAACAGGGCATTTGGTATTTGCGACCTTACACGCCAATAACGCCAACCAAGCGATTGATCGT
ATCATTCACTTTTTTGAAACCAGCCGCCACAACCAATTGTTTATGGACTTATCTCTTAACTTACAAGCTATTATTGCTCA
GCAATTGATTCCAACACCTGACGGTAAAGGACGCCGTGCCGCTATTGAGATTTTATTAAACTCTCAGCTGATTAGTGATT
ATATCCGTAAAGGCGAAATTCATGAAATCAAAGATGTCATGACGCGCTCACGCGATAGCGGCATGCAAACCTTTGATCAA
GCGCTCTTTGATTTATATGAAAATGGTGAGATTACTTATAAAGAAGCAATTCTCCATGCTGACTCTCCTAATGATTTGCG
CTTAAAAATTAAACTTAGCAGCAAAAATGGTAACGCTAATTTAGATGAAGGCGCCGATAGTTTGTCGTTAGATATCACTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A229GJH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.416

100

0.654
  pilU Acinetobacter baylyi ADP1

66.851

97.051

0.649
  pilU Vibrio cholerae strain A1552

52.174

98.66

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.803

98.123

0.41
  pilT Vibrio cholerae strain A1552

40.896

89.812

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.896

89.812

0.367
  pilT Acinetobacter nosocomialis M2

39.942

91.957

0.367
  pilT Acinetobacter baumannii D1279779

39.942

91.957

0.367
  pilT Acinetobacter baumannii strain A118

39.942

91.957

0.367
  pilT Legionella pneumophila strain Lp02

41.846

87.131

0.365
  pilT Legionella pneumophila strain ERS1305867

41.846

87.131

0.365

Multiple sequence alignment