Detailed information NTG1145336

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: DXE33_RS03965
• Genome accession: NZ_LT615228
• Coordinates: 759218..759925 (-)
• Length: 235 a.a.
• NCBI ID: WP_331851814.1
• Uniprot ID: -
• Organism: Polynucleobacter necessarius isolate PPGSP1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 754218..764925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DXE33_RS03930	-	754498..756888 (-)	2391	WP_114638703.1	PilC/PilY family type IV pilus protein	-
DXE33_RS03935	-	756892..757521 (-)	630	WP_114638704.1	hypothetical protein	-
DXE33_RS03945	-	757887..758192 (-)	306	WP_114638706.1	type II secretion system protein J	-
DXE33_RS03950	-	758189..758644 (-)	456	WP_114638707.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DXE33_RS03955	-	758660..758872 (-)	213	WP_162785402.1	hypothetical protein	-
DXE33_RS10665	-	758961..759221 (-)	261	WP_114638709.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DXE33_RS03965	pilT	759218..759925 (-)	708	WP_331851814.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DXE33_RS09785	-	759929..760219 (-)	291	WP_197711991.1	hypothetical protein	-
DXE33_RS03970	-	760270..761130 (-)	861	WP_114638710.1	A24 family peptidase	-
DXE33_RS03975	-	761151..761615 (-)	465	WP_114638711.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DXE33_RS03980	-	761713..762216 (-)	504	WP_114638712.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DXE33_RS09600	-	762423..762584 (-)	162	WP_162785403.1	hypothetical protein	-
DXE33_RS03985	-	762661..762921 (-)	261	WP_114638713.1	DUF4124 domain-containing protein	-
DXE33_RS03990	-	762924..763235 (-)	312	WP_114638714.1	PilZ domain-containing protein	-
DXE33_RS03995	-	763389..764165 (-)	777	WP_162785404.1	bifunctional diguanylate cyclase/phosphodiesterase	-
DXE33_RS10670	-	764162..764365 (-)	204	WP_114638716.1	diguanylate cyclase domain-containing protein	-

Sequence

Protein

• Length: 235 a.a.
• Molecular weight: 26027.01 Da
• Isoelectric Point: 6.3429

>NTDB_id=1145336 DXE33_RS03965 WP_331851814.1 759218..759925(-) (pilT) [Polynucleobacter necessarius isolate PPGSP1]
METQIPAFDVLGLPPVARDVIELENGLILVTGPTGSGKSTTLAAMIDRINRTRHEHIITIEDPIEFIHQNQKCVVSQREV
KRDTLSFSSALRASLREDPDVILVGELRDLETIQLALTAAETGHLVFGTLHTSGAPNTINRIIDVFPPQQQDQVRAQLSQ
SLRLVMTQRLLKRKDAPGRIGAFEVMTCNPAVRNLIRENKVFQIPSVMQMARGEGMMTMEASLQQLVQSGQIEAT

Nucleotide

• Length: 708 bp

>NTDB_id=1145336 DXE33_RS03965 WP_331851814.1 759218..759925(-) (pilT) [Polynucleobacter necessarius isolate PPGSP1]
ATTGAGACCCAAATTCCTGCGTTTGACGTGTTGGGTTTGCCCCCAGTAGCCAGAGATGTAATTGAGTTGGAGAATGGCCT
CATTCTGGTTACTGGCCCGACAGGCTCTGGTAAATCAACCACATTAGCGGCCATGATTGATCGCATTAATCGCACTCGTC
ATGAGCACATCATTACCATTGAAGATCCTATTGAGTTTATTCATCAAAATCAGAAATGCGTTGTTTCGCAGCGTGAGGTC
AAGCGAGATACCTTATCTTTTTCATCCGCATTGCGAGCCTCATTGCGTGAAGATCCTGATGTTATTTTGGTTGGGGAGTT
GCGTGACTTAGAGACCATTCAATTGGCACTCACTGCCGCTGAAACAGGTCACTTGGTTTTTGGGACCTTACATACTAGTG
GCGCCCCAAATACGATTAACCGAATCATCGACGTATTTCCACCGCAACAGCAAGATCAAGTGAGGGCGCAACTTTCACAA
TCCTTACGCCTGGTAATGACGCAGCGCCTTCTCAAACGAAAAGATGCGCCAGGTCGAATTGGCGCTTTTGAAGTGATGAC
TTGTAATCCTGCAGTGCGTAACTTAATTCGTGAAAATAAAGTTTTCCAAATTCCTAGTGTGATGCAGATGGCTCGTGGCG
AAGGCATGATGACCATGGAAGCCTCTTTGCAGCAGCTGGTTCAGTCCGGTCAAATTGAGGCGACATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (19-172)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	59.914	98.723	0.591
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	59.914	98.723	0.591
pilT	Legionella pneumophila strain Lp02	60	97.872	0.587
pilT	Legionella pneumophila strain ERS1305867	60	97.872	0.587
pilT	Acinetobacter baylyi ADP1	60	97.872	0.587
pilT	Acinetobacter baumannii D1279779	61.261	94.468	0.579
pilT	Acinetobacter baumannii strain A118	61.261	94.468	0.579
pilT	Acinetobacter nosocomialis M2	59.13	97.872	0.579
pilT	Neisseria meningitidis 8013	59.735	96.17	0.574
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	61.927	92.766	0.574
pilT	Neisseria gonorrhoeae MS11	59.292	96.17	0.57
pilT	Pseudomonas stutzeri DSM 10701	57.826	97.872	0.566
pilT	Pseudomonas aeruginosa PAK	57.826	97.872	0.566
pilU	Vibrio cholerae strain A1552	44.828	98.723	0.443
pilU	Pseudomonas stutzeri DSM 10701	44.828	98.723	0.443
pilU	Acinetobacter baylyi ADP1	43.103	98.723	0.426
pilB	Legionella pneumophila strain ERS1305867	32.841	100	0.379
pilB	Vibrio cholerae strain A1552	32.197	100	0.362