Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NCTC9997_RS12275 Genome accession   NZ_LT575468
Coordinates   2754461..2755561 (-) Length   366 a.a.
NCBI ID   WP_010864554.1    Uniprot ID   R8AMI2
Organism   Plesiomonas shigelloides strain NCTC10360     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2749461..2760561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCTC9997_RS12245 (BAY29_RS12110) rsmE 2749922..2750656 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NCTC9997_RS12250 (BAY29_RS12115) gshB 2750666..2751619 (+) 954 WP_047708158.1 glutathione synthase -
  NCTC9997_RS12255 (BAY29_RS12120) - 2751833..2752396 (+) 564 WP_064978164.1 YqgE/AlgH family protein -
  NCTC9997_RS12260 (BAY29_RS12125) ruvX 2752421..2752840 (+) 420 WP_064978165.1 Holliday junction resolvase RuvX -
  NCTC9997_RS12265 (BAY29_RS12130) npr 2752857..2753132 (-) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  NCTC9997_RS12270 (BAY29_RS12135) pilU 2753368..2754477 (-) 1110 WP_010864553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NCTC9997_RS12275 (BAY29_RS12140) pilT 2754461..2755561 (-) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  NCTC9997_RS12280 (BAY29_RS12145) - 2755584..2756291 (+) 708 WP_039045867.1 YggS family pyridoxal phosphate-dependent enzyme -
  NCTC9997_RS12285 (BAY29_RS12150) proC 2756374..2757192 (+) 819 WP_064978166.1 pyrroline-5-carboxylate reductase -
  NCTC9997_RS12290 (BAY29_RS12155) - 2757221..2757775 (+) 555 WP_010864557.1 YggT family protein -
  NCTC9997_RS12295 (BAY29_RS12160) yggU 2757787..2758074 (+) 288 WP_010864558.1 DUF167 family protein YggU -
  NCTC9997_RS12300 (BAY29_RS12165) - 2758143..2758646 (+) 504 WP_082935559.1 DUF4426 domain-containing protein -
  NCTC9997_RS12305 (BAY29_RS12170) - 2758840..2759445 (+) 606 WP_064978167.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40421.07 Da        Isoelectric Point: 6.7432

>NTDB_id=1143894 NCTC9997_RS12275 WP_010864554.1 2754461..2755561(-) (pilT) [Plesiomonas shigelloides strain NCTC10360]
MDITELLAFSVKHNASDLHLSAGNPPLIRVDGEVRRVNMPVLDGREVRRLVYDIMNDVQRRDFETEMELDFSFELNGVGR
FRVNAYQQQRGISAALRTIPTDIPTLEQLNAPEVFRNIAMQPRGLVLVTGPTGSGKSTTLAAMLNYVNQHMHHHILTIED
PIEFIHTSNKCLINQREVHRHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGAEKDMVRSMLSESLKAVISQVLLRKEGGGRVAAHEIMLGTPAIRNLIREDKIHQIYSILQTSSRQGMQTLEQAL
NVLTSQGVVSYEEAQRKMNSLMDGGIQNGNTAGGTGAGAMGYGFAG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1143894 NCTC9997_RS12275 WP_010864554.1 2754461..2755561(-) (pilT) [Plesiomonas shigelloides strain NCTC10360]
ATGGACATAACAGAACTGTTGGCTTTTAGTGTAAAACATAATGCCTCGGATCTGCACCTATCTGCCGGTAACCCACCGCT
GATCCGCGTCGACGGAGAAGTGCGTCGGGTCAATATGCCGGTCCTGGATGGCCGTGAAGTGCGCCGTCTGGTGTATGACA
TTATGAACGATGTTCAGCGCCGTGACTTTGAAACTGAGATGGAACTGGACTTTTCGTTCGAACTGAACGGGGTTGGCCGT
TTCCGGGTTAATGCTTATCAGCAGCAACGGGGAATTTCGGCGGCACTGCGTACCATACCTACAGATATACCAACACTGGA
GCAGTTAAACGCACCGGAAGTGTTTCGGAATATTGCCATGCAGCCGCGTGGATTGGTATTGGTGACCGGCCCGACCGGCT
CGGGGAAGTCGACCACCTTGGCGGCGATGCTCAATTATGTCAATCAGCACATGCATCACCACATTCTGACCATTGAAGAT
CCGATAGAATTTATCCACACCAGTAACAAGTGCCTGATTAACCAGCGTGAGGTGCATCGGCATACCCACAGTTTTAATAA
TGCACTGCGCTCGGCGCTGCGTGAAGACCCGGATGTGATTTTGGTGGGTGAGCTGCGCGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCCGAAACCGGCCACTTGGTGTTTGGCACCTTGCACACCAGCTCGGCGGCCAAAACCATCGACCGTATT
GTGGATGTGTTCCCCGGCGCGGAAAAAGACATGGTGCGCTCGATGCTGTCTGAATCGCTCAAAGCGGTTATCTCGCAGGT
ATTGCTGCGTAAAGAAGGCGGCGGACGTGTGGCGGCGCACGAGATCATGCTGGGAACGCCAGCTATTCGTAACTTGATCC
GTGAAGATAAAATTCACCAGATCTATTCGATTTTGCAGACCAGCTCGCGTCAAGGTATGCAGACGTTGGAGCAGGCGCTG
AATGTGCTGACCAGTCAGGGCGTGGTCAGCTACGAAGAGGCGCAGCGCAAGATGAACTCGTTGATGGATGGCGGGATCCA
AAACGGCAATACCGCGGGTGGCACCGGTGCAGGAGCGATGGGCTATGGTTTCGCTGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.373

92.35

0.678
  pilT Vibrio cholerae strain A1552

73.373

92.35

0.678
  pilT Pseudomonas stutzeri DSM 10701

71.217

92.077

0.656
  pilT Acinetobacter nosocomialis M2

71.343

91.53

0.653
  pilT Acinetobacter baumannii D1279779

71.045

91.53

0.65
  pilT Acinetobacter baumannii strain A118

71.045

91.53

0.65
  pilT Pseudomonas aeruginosa PAK

70.623

92.077

0.65
  pilT Acinetobacter baylyi ADP1

70.448

91.53

0.645
  pilT Neisseria meningitidis 8013

64.793

92.35

0.598
  pilT Neisseria gonorrhoeae MS11

64.497

92.35

0.596
  pilT Legionella pneumophila strain Lp02

65.269

91.257

0.596
  pilT Legionella pneumophila strain ERS1305867

65.269

91.257

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.025

98.087

0.481
  pilU Vibrio cholerae strain A1552

43.023

93.989

0.404
  pilU Pseudomonas stutzeri DSM 10701

43.284

91.53

0.396
  pilU Acinetobacter baylyi ADP1

42.274

93.716

0.396

Multiple sequence alignment