Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NCTC9997_RS12270 Genome accession   NZ_LT575468
Coordinates   2753368..2754477 (-) Length   369 a.a.
NCBI ID   WP_010864553.1    Uniprot ID   R8AMH9
Organism   Plesiomonas shigelloides strain NCTC10360     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748368..2759477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCTC9997_RS12235 (BAY29_RS12100) - 2749022..2749231 (-) 210 WP_010864545.1 DUF1656 domain-containing protein -
  NCTC9997_RS12240 (BAY29_RS12105) - 2749228..2749740 (-) 513 WP_010864546.1 MarR family winged helix-turn-helix transcriptional regulator -
  NCTC9997_RS12245 (BAY29_RS12110) rsmE 2749922..2750656 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NCTC9997_RS12250 (BAY29_RS12115) gshB 2750666..2751619 (+) 954 WP_047708158.1 glutathione synthase -
  NCTC9997_RS12255 (BAY29_RS12120) - 2751833..2752396 (+) 564 WP_064978164.1 YqgE/AlgH family protein -
  NCTC9997_RS12260 (BAY29_RS12125) ruvX 2752421..2752840 (+) 420 WP_064978165.1 Holliday junction resolvase RuvX -
  NCTC9997_RS12265 (BAY29_RS12130) npr 2752857..2753132 (-) 276 WP_010864552.1 PTS phosphocarrier protein NPr -
  NCTC9997_RS12270 (BAY29_RS12135) pilU 2753368..2754477 (-) 1110 WP_010864553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NCTC9997_RS12275 (BAY29_RS12140) pilT 2754461..2755561 (-) 1101 WP_010864554.1 type IV pilus twitching motility protein PilT Machinery gene
  NCTC9997_RS12280 (BAY29_RS12145) - 2755584..2756291 (+) 708 WP_039045867.1 YggS family pyridoxal phosphate-dependent enzyme -
  NCTC9997_RS12285 (BAY29_RS12150) proC 2756374..2757192 (+) 819 WP_064978166.1 pyrroline-5-carboxylate reductase -
  NCTC9997_RS12290 (BAY29_RS12155) - 2757221..2757775 (+) 555 WP_010864557.1 YggT family protein -
  NCTC9997_RS12295 (BAY29_RS12160) yggU 2757787..2758074 (+) 288 WP_010864558.1 DUF167 family protein YggU -
  NCTC9997_RS12300 (BAY29_RS12165) - 2758143..2758646 (+) 504 WP_082935559.1 DUF4426 domain-containing protein -
  NCTC9997_RS12305 (BAY29_RS12170) - 2758840..2759445 (+) 606 WP_064978167.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41485.52 Da        Isoelectric Point: 5.7325

>NTDB_id=1143893 NCTC9997_RS12270 WP_010864553.1 2753368..2754477(-) (pilU) [Plesiomonas shigelloides strain NCTC10360]
MVSLDELLQEMLRQQASDLYVSVDVPAQLRINHELHPLGEALSIETVTELVAQAMGAERFLQFCQQKEGNMALVRPFARF
RVSGFWQRELPGMVIRHIRTDIPTIEELGLPMLTGELAMTRKGLILVVGATGSGKTTTLAAMLGYRNEFDHSHILTVEDP
IEFIHPHKRCLITQREVGMDTESYEVALRNALRQAPDVIQVGEIRTRDTLQHAIGFSETGHLCIATLHANNASQALERMV
HMIPEGSREAFLFDLSNNLRAIIGQQLVPGITPGRRLLVHEILINTPRVTDLLRKNQLYELRDVMASNSEIGMCTFDQSL
YGLYRRGLISYTDALHYAESANDLRLRIRSSGDEPFSPNTFDDVTVDLS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1143893 NCTC9997_RS12270 WP_010864553.1 2753368..2754477(-) (pilU) [Plesiomonas shigelloides strain NCTC10360]
ATGGTTTCGCTGGATGAGTTATTGCAGGAGATGCTGCGCCAGCAGGCGTCGGATCTGTATGTTTCGGTCGATGTGCCCGC
GCAGCTGCGCATCAATCATGAGCTGCACCCACTAGGTGAGGCGCTGTCGATTGAAACGGTCACCGAGCTGGTGGCGCAGG
CGATGGGCGCCGAGCGCTTCTTACAGTTTTGTCAGCAAAAAGAGGGCAACATGGCGCTGGTGCGTCCGTTTGCCCGTTTT
CGGGTCTCCGGTTTTTGGCAGCGTGAATTGCCGGGCATGGTGATCCGTCATATTCGAACCGATATTCCGACCATTGAAGA
GCTGGGCTTACCGATGTTAACCGGTGAGCTGGCGATGACGCGCAAAGGTTTGATTTTGGTGGTCGGCGCTACCGGCAGCG
GGAAAACCACCACGCTGGCAGCGATGCTCGGCTATCGCAACGAGTTTGACCATTCGCATATTCTGACGGTGGAAGATCCG
ATTGAATTTATCCATCCGCATAAACGCTGTTTAATCACCCAGCGTGAAGTGGGGATGGATACCGAAAGCTACGAAGTCGC
CTTGCGTAACGCGCTGCGCCAAGCACCGGATGTGATTCAGGTTGGCGAGATCCGCACCCGCGACACGCTGCAACATGCGA
TTGGCTTTTCCGAAACCGGTCATCTGTGTATCGCCACCTTGCACGCCAATAACGCCAGCCAAGCGCTGGAGCGGATGGTA
CACATGATCCCTGAAGGCAGCCGCGAAGCTTTCTTGTTTGATTTATCCAACAACTTGCGCGCCATTATCGGGCAGCAGCT
GGTGCCGGGGATCACCCCAGGGCGCCGTTTACTGGTGCACGAGATTTTGATCAACACGCCGCGCGTGACCGATTTGCTGC
GTAAAAATCAGTTGTATGAGCTGCGTGATGTGATGGCCAGTAACAGCGAGATTGGCATGTGCACCTTTGACCAATCGCTG
TACGGCTTGTATCGGCGCGGGCTGATTAGCTATACCGATGCGCTGCATTATGCGGAGTCGGCCAATGATTTGCGCTTGCG
GATCCGCAGTAGCGGGGATGAGCCGTTCTCACCGAACACCTTCGATGATGTGACGGTGGATTTAAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8AMH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

54.768

99.458

0.545
  pilU Pseudomonas stutzeri DSM 10701

51.275

95.664

0.491
  pilU Acinetobacter baylyi ADP1

46.742

95.664

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

99.187

0.39

Multiple sequence alignment