Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AC2117_RS14345 Genome accession   NZ_LS999521
Coordinates   2998367..2999404 (+) Length   345 a.a.
NCBI ID   WP_009390483.1    Uniprot ID   K9A7G6
Organism   Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2993367..3004404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC2117_RS14330 (AC2117_02845) - 2996087..2996482 (-) 396 WP_133975017.1 hypothetical protein -
  AC2117_RS14335 (AC2117_02846) - 2996647..2997384 (-) 738 WP_133975019.1 hypothetical protein -
  AC2117_RS14340 (AC2117_02847) - 2997554..2998240 (-) 687 WP_133975021.1 YggS family pyridoxal phosphate-dependent enzyme -
  AC2117_RS14345 (AC2117_02848) pilT 2998367..2999404 (+) 1038 WP_009390483.1 type IV pilus twitching motility protein PilT Machinery gene
  AC2117_RS14350 (AC2117_02849) pilU 2999424..3000542 (+) 1119 WP_133975023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AC2117_RS14355 (AC2117_02850) fur 3000612..3001049 (-) 438 WP_133975025.1 ferric iron uptake transcriptional regulator -
  AC2117_RS14360 (AC2117_02851) - 3001161..3001559 (+) 399 WP_003653245.1 outer membrane protein assembly factor BamE -
  AC2117_RS14365 (AC2117_02852) - 3001627..3001938 (-) 312 WP_132072003.1 RnfH family protein -
  AC2117_RS14370 (AC2117_02853) - 3001938..3003005 (-) 1068 WP_133975027.1 hypothetical protein -
  AC2117_RS14380 (AC2117_02854) - 3003160..3003615 (-) 456 WP_133975029.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38332.92 Da        Isoelectric Point: 6.7269

>NTDB_id=1143497 AC2117_RS14345 WP_009390483.1 2998367..2999404(+) (pilT) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSSIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1143497 AC2117_RS14345 WP_009390483.1 2998367..2999404(+) (pilT) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
ATGGATATTACAGAGCTACTCGCTTTCTCTGTGAAAAATGGCGCGTCCGATTTACATTTGTCTGCTGGCATGCCACCAAT
GATTCGCGTGGATGGCGAAGTCCGCCGCATTAACCTTCCAGCTCTAGACCATAAAGATGTTCACCGTCTGGTCTATGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAGCTCGAAACAGACTTTTCTTTTGAAGTGCCAAATGTTGCGCGT
TTTCGTGTCAATGCATTTAACCAAAACCGTGGTGCCGGAGCCGTTTTTCGTACCATTCCATCAAAAGTACTCACAATGGA
AGATTTAGGCTTAGGGCAAATCTTTAAAGATATTTGTGATTACCCACGTGGCATTGTGTTAGTTACAGGACCAACAGGTT
CAGGTAAATCAACCACGCTTGCAGCGATGATGGACTATATTAACGAAAACCGTTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTCCATCAGTCTAAAAAATGTCTGATTAACCAACGTGAAGTACACCGCGACACGCATGGCTTTAACGA
AGCACTACGCTCAGCACTGCGTGAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTAG
CACTTACCGCTGCTGAAACTGGTCACTTGGTGTTTGGTACGCTCCATACGACTTCTGCTGCAAAAACCATTGACCGTGTG
ATTGATGTATTCCCTGCCGAAGAAAAAGACATGGTGCGTGCCATGTTGTCAGAATCTTTACAGGCTGTTATTTCTCAAAC
TCTACTTAAAAAGAATGGCGGTGGGCGTGTCGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAAGTTGCCCAAATGTATTCATCAATTCAGACGGGCGCTAATCATGGCATGACCACGCTCGACCAAAGCTTA
AAAGGTCTAGTTGCTCGTGGGGTGATTAGCCCACAAACGGCACGTACTGCCGCAAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9A7G6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter nosocomialis M2

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

89.244

99.71

0.89
  pilT Pseudomonas stutzeri DSM 10701

84.302

99.71

0.841
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment