Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AC2117_RS14350 Genome accession   NZ_LS999521
Coordinates   2999424..3000542 (+) Length   372 a.a.
NCBI ID   WP_133975023.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2994424..3005542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC2117_RS14330 (AC2117_02845) - 2996087..2996482 (-) 396 WP_133975017.1 hypothetical protein -
  AC2117_RS14335 (AC2117_02846) - 2996647..2997384 (-) 738 WP_133975019.1 hypothetical protein -
  AC2117_RS14340 (AC2117_02847) - 2997554..2998240 (-) 687 WP_133975021.1 YggS family pyridoxal phosphate-dependent enzyme -
  AC2117_RS14345 (AC2117_02848) pilT 2998367..2999404 (+) 1038 WP_009390483.1 type IV pilus twitching motility protein PilT Machinery gene
  AC2117_RS14350 (AC2117_02849) pilU 2999424..3000542 (+) 1119 WP_133975023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AC2117_RS14355 (AC2117_02850) fur 3000612..3001049 (-) 438 WP_133975025.1 ferric iron uptake transcriptional regulator -
  AC2117_RS14360 (AC2117_02851) - 3001161..3001559 (+) 399 WP_003653245.1 outer membrane protein assembly factor BamE -
  AC2117_RS14365 (AC2117_02852) - 3001627..3001938 (-) 312 WP_132072003.1 RnfH family protein -
  AC2117_RS14370 (AC2117_02853) - 3001938..3003005 (-) 1068 WP_133975027.1 hypothetical protein -
  AC2117_RS14380 (AC2117_02854) - 3003160..3003615 (-) 456 WP_133975029.1 bacteriohemerythrin -
  AC2117_RS14385 (AC2117_02855) - 3003867..3004715 (-) 849 WP_133976384.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41830.99 Da        Isoelectric Point: 6.5053

>NTDB_id=1143498 AC2117_RS14350 WP_133975023.1 2999424..3000542(+) (pilU) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGDVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLVPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDQQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1143498 AC2117_RS14350 WP_133975023.1 2999424..3000542(+) (pilU) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
ATGGATTTTAACGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCGCCATC
TATGAAAATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGACGTTATTGGTCAACTTTTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACCGCC
CGCTTTCGTGTGAGCGCTTTTCAGCAGCGTGACATGCCAGGCATGGTGCTACGCCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTTCTTAAAGATTTATCAATGACCAAACGTGGCATCATTATTTTTGTTGGTGCAACAG
GTACGGGTAAATCGACGTCTTTGGCTTCAATGATCAGTTATCGTAATCACAATACTAAAGGTCATATCATTACCATTGAA
GACCCAATCGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACAGACTCATTTGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCAGATGTCATCTTGATTGGTGAGATTCGCTCTCGTGAAGTGATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTGTTAGCCACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTTTACATGGACTTATCGCTTAACTTAAAAGCCATGATTGCGCA
GCAACTTGTTCCAACACCAGATGGTAATTCTCGCCGCGCCGCAATTGAAATTTTAATTAACTCGCCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAGCGTTCGCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCCTATTTGACCTCTATAAAGCAGGCCAAATTACCTACAAAGATGCGTTAAAACATGCCGACTCACCTAACGATTTACG
TTTGACGATTAAGCTTGCCGAAGAAGGACCTGATCAAATTGCGGGCACAAATCAGCATTTAACCTTCGACCAACAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(32-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

74.868

100

0.761
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.731

0.677
  pilU Vibrio cholerae strain A1552

53.763

100

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.659

97.849

0.398
  pilT Legionella pneumophila strain Lp02

39.645

90.86

0.36
  pilT Legionella pneumophila strain ERS1305867

39.645

90.86

0.36

Multiple sequence alignment