Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D5R51_RS11220 Genome accession   NZ_LS997867
Coordinates   2434403..2435440 (-) Length   345 a.a.
NCBI ID   WP_119091522.1    Uniprot ID   -
Organism   Vibrio paracholerae strain NCTC 30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2429403..2440440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5R51_RS11195 (SAMEA104470976_02233) - 2429887..2430450 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  D5R51_RS11200 (SAMEA104470976_02234) ruvX 2430531..2430953 (+) 423 WP_029627830.1 Holliday junction resolvase RuvX -
  D5R51_RS11205 (SAMEA104470976_02235) tyrS 2430978..2432258 (-) 1281 WP_162892015.1 tyrosine--tRNA ligase -
  D5R51_RS11210 (SAMEA104470976_02236) - 2432367..2433164 (-) 798 WP_162892016.1 helix-turn-helix transcriptional regulator -
  D5R51_RS11215 (SAMEA104470976_02237) pilU 2433282..2434388 (-) 1107 WP_113601003.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D5R51_RS11220 (SAMEA104470976_02238) pilT 2434403..2435440 (-) 1038 WP_119091522.1 type IV pilus twitching motility protein PilT Machinery gene
  D5R51_RS11225 (SAMEA104470976_02239) - 2435466..2436176 (+) 711 WP_119091523.1 YggS family pyridoxal phosphate-dependent enzyme -
  D5R51_RS11230 (SAMEA104470976_02240) proC 2436229..2437047 (+) 819 WP_119091524.1 pyrroline-5-carboxylate reductase -
  D5R51_RS11235 (SAMEA104470976_02241) - 2437101..2437658 (+) 558 WP_001087261.1 YggT family protein -
  D5R51_RS11240 (SAMEA104470976_02242) yggU 2437658..2437948 (+) 291 WP_000718489.1 DUF167 family protein YggU -
  D5R51_RS11245 (SAMEA104470976_02243) - 2437994..2438425 (+) 432 WP_001233673.1 DUF4426 domain-containing protein -
  D5R51_RS11250 (SAMEA104470976_02244) - 2438495..2439097 (+) 603 WP_000725014.1 XTP/dITP diphosphatase -
  D5R51_RS11255 (SAMEA104470976_02245) hemW 2439091..2440266 (+) 1176 WP_119091525.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38220.80 Da        Isoelectric Point: 6.6672

>NTDB_id=1143356 D5R51_RS11220 WP_119091522.1 2434403..2435440(-) (pilT) [Vibrio paracholerae strain NCTC 30]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDGQRSEYEEKLEVDFSFELPSVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTTAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1143356 D5R51_RS11220 WP_119091522.1 2434403..2435440(-) (pilT) [Vibrio paracholerae strain NCTC 30]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACACCGTTTGATTTTTGAGA
TTATGAACGATGGTCAACGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAGTGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTTATCCCGACGTTAGA
GCAATTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CAGGAAAGTCCACAACTTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAGCGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGTTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCACGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
GCTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCGCGTGGTGTGGTGGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.13

100

0.991
  pilT Vibrio cholerae strain A1552

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

71.131

97.391

0.693
  pilT Acinetobacter baumannii strain A118

71.131

97.391

0.693
  pilT Acinetobacter nosocomialis M2

70.833

97.391

0.69
  pilT Acinetobacter baylyi ADP1

71.818

95.652

0.687
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

63.939

95.652

0.612
  pilT Neisseria gonorrhoeae MS11

63.636

95.652

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.682

94.783

0.49
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment