Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   D5R51_RS11215 Genome accession   NZ_LS997867
Coordinates   2433282..2434388 (-) Length   368 a.a.
NCBI ID   WP_113601003.1    Uniprot ID   -
Organism   Vibrio paracholerae strain NCTC 30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2428282..2439388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5R51_RS11190 (SAMEA104470976_02232) gshB 2428895..2429851 (+) 957 WP_000593313.1 glutathione synthase -
  D5R51_RS11195 (SAMEA104470976_02233) - 2429887..2430450 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  D5R51_RS11200 (SAMEA104470976_02234) ruvX 2430531..2430953 (+) 423 WP_029627830.1 Holliday junction resolvase RuvX -
  D5R51_RS11205 (SAMEA104470976_02235) tyrS 2430978..2432258 (-) 1281 WP_162892015.1 tyrosine--tRNA ligase -
  D5R51_RS11210 (SAMEA104470976_02236) - 2432367..2433164 (-) 798 WP_162892016.1 helix-turn-helix transcriptional regulator -
  D5R51_RS11215 (SAMEA104470976_02237) pilU 2433282..2434388 (-) 1107 WP_113601003.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D5R51_RS11220 (SAMEA104470976_02238) pilT 2434403..2435440 (-) 1038 WP_119091522.1 type IV pilus twitching motility protein PilT Machinery gene
  D5R51_RS11225 (SAMEA104470976_02239) - 2435466..2436176 (+) 711 WP_119091523.1 YggS family pyridoxal phosphate-dependent enzyme -
  D5R51_RS11230 (SAMEA104470976_02240) proC 2436229..2437047 (+) 819 WP_119091524.1 pyrroline-5-carboxylate reductase -
  D5R51_RS11235 (SAMEA104470976_02241) - 2437101..2437658 (+) 558 WP_001087261.1 YggT family protein -
  D5R51_RS11240 (SAMEA104470976_02242) yggU 2437658..2437948 (+) 291 WP_000718489.1 DUF167 family protein YggU -
  D5R51_RS11245 (SAMEA104470976_02243) - 2437994..2438425 (+) 432 WP_001233673.1 DUF4426 domain-containing protein -
  D5R51_RS11250 (SAMEA104470976_02244) - 2438495..2439097 (+) 603 WP_000725014.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41327.30 Da        Isoelectric Point: 6.5801

>NTDB_id=1143355 D5R51_RS11215 WP_113601003.1 2433282..2434388(-) (pilU) [Vibrio paracholerae strain NCTC 30]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLQAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEILLNSPRISDLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1143355 D5R51_RS11215 WP_113601003.1 2433282..2434388(-) (pilU) [Vibrio paracholerae strain NCTC 30]
ATGGAGTTGAATCAATACCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATCACTGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCAGGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCGACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCAATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGCGAAATTCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACTTTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGGATTTTGCAT
CTGGTGCCCAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCTCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAATTCTGCTCAATAGCCCGCGCATTTCCGATTTGATCCGCC
GTGGCGAGCTACACGAGCTAAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCTTTGTAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCTAACGATCTGCGTCTGATGCT
CAAAACCAAGCGTGGTGATAACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

98.913

100

0.989
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

43.068

92.12

0.397
  pilT Legionella pneumophila strain Lp02

43.068

92.12

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Vibrio cholerae strain A1552

42.515

90.761

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

90.761

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

42.769

88.315

0.378
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.617

94.293

0.364

Multiple sequence alignment