Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SHEWBE_RS07620 Genome accession   NZ_LS483452
Coordinates   1643299..1644336 (+) Length   345 a.a.
NCBI ID   WP_112352165.1    Uniprot ID   A0A330M0V9
Organism   Shewanella benthica strain DB21MT-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1638299..1649336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWBE_RS07590 (SHEWBE_1769) hemW 1638394..1639530 (-) 1137 WP_112352161.1 radical SAM family heme chaperone HemW -
  SHEWBE_RS07595 (SHEWBE_1770) rdgB 1639625..1640227 (-) 603 WP_112352162.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHEWBE_RS07600 (SHEWBE_1771) - 1640378..1640812 (-) 435 WP_005497515.1 DUF4426 domain-containing protein -
  SHEWBE_RS07605 (SHEWBE_1772) - 1640985..1641533 (-) 549 WP_112352163.1 YggT family protein -
  SHEWBE_RS07610 (SHEWBE_1773) proC 1641609..1642427 (-) 819 WP_112352164.1 pyrroline-5-carboxylate reductase -
  SHEWBE_RS07615 (SHEWBE_1774) - 1642567..1643262 (-) 696 WP_112354086.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEWBE_RS07620 (SHEWBE_1775) pilT 1643299..1644336 (+) 1038 WP_112352165.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEWBE_RS07625 (SHEWBE_1776) pilU 1644360..1645472 (+) 1113 WP_112352166.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEWBE_RS07630 (SHEWBE_1777) - 1645646..1646743 (+) 1098 WP_231926475.1 hypothetical protein -
  SHEWBE_RS07635 (SHEWBE_1778) ruvX 1646862..1647284 (-) 423 WP_112352168.1 Holliday junction resolvase RuvX -
  SHEWBE_RS07640 (SHEWBE_1779) - 1647322..1647882 (-) 561 WP_112352169.1 YqgE/AlgH family protein -
  SHEWBE_RS07645 (SHEWBE_1780) yciH 1647950..1648279 (-) 330 WP_112352170.1 stress response translation initiation inhibitor YciH -
  SHEWBE_RS07650 (SHEWBE_1781) - 1648458..1648775 (-) 318 WP_231926477.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38376.94 Da        Isoelectric Point: 6.9449

>NTDB_id=1142118 SHEWBE_RS07620 WP_112352165.1 1643299..1644336(+) (pilT) [Shewanella benthica strain DB21MT-2]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHGLIYDIMNDKQRKDYEEHLEIDFSFEIPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKQIASFPRGLVLVTGPTGSGKSTTLAAMVDYINESRHEHILTIED
PIEFVHQSKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
QNLVNRGQISREDAQHKSANKQTKF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1142118 SHEWBE_RS07620 WP_112352165.1 1643299..1644336(+) (pilT) [Shewanella benthica strain DB21MT-2]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAGCACAAAGCGTCCGATCTACACCTTTCTGCTGGTGTTTCGCCTAT
GATACGTGTTGATGGCGAAGTCAGAAAGATCAATTTACCCGCTTTAGATCATCAAGGGGTGCATGGACTCATCTATGACA
TCATGAATGATAAGCAACGTAAAGATTATGAAGAACATTTAGAGATAGATTTCTCATTCGAAATACCCAACTTAGCACGT
TTTCGTGTCAATGCCTTCAACCAATCGCGAGGAGCCGCTGCGGTATTTCGTACCATTCCCAGCGATATTTTGAGTTTGGA
GCAGCTTGGCGCTCCGGAGATTTTTAAGCAAATTGCCAGCTTTCCACGTGGCTTAGTCCTGGTTACCGGTCCAACAGGCT
CGGGAAAGAGTACTACTTTGGCTGCCATGGTTGACTATATTAATGAAAGCCGTCACGAACACATTCTCACCATCGAAGAC
CCAATCGAATTCGTCCATCAGAGCAAGCAATGTCTGATCAATCAGCGAGAGGTGCATCGTCATACTCATAGTTTTAACGC
GGCCTTGCGAAGCGCGTTGCGTGAAGATCCCGATGTTATCTTAGTAGGCGAGATGCGTGATCTTGAAACCATCAGGCTGG
CGATGACGGCAGCTGAAACGGGTCATCTGGTTTTCGGTACTTTACACACCACCTCAGCGGCAAAAACCATAGATCGTGTG
GTCGATGTTTTCCCCGAAGGCGAGAAGGGCATGGTCAGAACCATGTTATCTGAGTCGTTACAGGCGGTAATATCACAGAC
CTTGATCAAGAAAATCGGTGGCGGACGTGTGGCGGCCCATGAGATCATGATGGGAACTCCAGCGATCAGAAACCTTATCC
GTGAGGATAAAGTGGCGCAGATGTACTCTGCGATTCAGACCGGTATGTCACATGGTATGCAGACATTAGATCAATGCCTG
CAGAACTTGGTCAACCGCGGTCAGATCAGTCGTGAAGATGCTCAGCATAAGAGTGCCAATAAACAGACAAAATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A330M0V9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Pseudomonas stutzeri DSM 10701

78.402

97.971

0.768
  pilT Acinetobacter baylyi ADP1

78.806

97.101

0.765
  pilT Legionella pneumophila strain Lp02

76.833

98.841

0.759
  pilT Legionella pneumophila strain ERS1305867

76.833

98.841

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.681

0.704
  pilT Vibrio cholerae strain A1552

72.107

97.681

0.704
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.07

94.783

0.484
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.593

98.261

0.409
  pilU Acinetobacter baylyi ADP1

40.774

97.391

0.397

Multiple sequence alignment