Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SHEWBE_RS07625 Genome accession   NZ_LS483452
Coordinates   1644360..1645472 (+) Length   370 a.a.
NCBI ID   WP_112352166.1    Uniprot ID   A0A330M0G8
Organism   Shewanella benthica strain DB21MT-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1639360..1650472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWBE_RS07595 (SHEWBE_1770) rdgB 1639625..1640227 (-) 603 WP_112352162.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHEWBE_RS07600 (SHEWBE_1771) - 1640378..1640812 (-) 435 WP_005497515.1 DUF4426 domain-containing protein -
  SHEWBE_RS07605 (SHEWBE_1772) - 1640985..1641533 (-) 549 WP_112352163.1 YggT family protein -
  SHEWBE_RS07610 (SHEWBE_1773) proC 1641609..1642427 (-) 819 WP_112352164.1 pyrroline-5-carboxylate reductase -
  SHEWBE_RS07615 (SHEWBE_1774) - 1642567..1643262 (-) 696 WP_112354086.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEWBE_RS07620 (SHEWBE_1775) pilT 1643299..1644336 (+) 1038 WP_112352165.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEWBE_RS07625 (SHEWBE_1776) pilU 1644360..1645472 (+) 1113 WP_112352166.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEWBE_RS07630 (SHEWBE_1777) - 1645646..1646743 (+) 1098 WP_231926475.1 hypothetical protein -
  SHEWBE_RS07635 (SHEWBE_1778) ruvX 1646862..1647284 (-) 423 WP_112352168.1 Holliday junction resolvase RuvX -
  SHEWBE_RS07640 (SHEWBE_1779) - 1647322..1647882 (-) 561 WP_112352169.1 YqgE/AlgH family protein -
  SHEWBE_RS07645 (SHEWBE_1780) yciH 1647950..1648279 (-) 330 WP_112352170.1 stress response translation initiation inhibitor YciH -
  SHEWBE_RS07650 (SHEWBE_1781) - 1648458..1648775 (-) 318 WP_231926477.1 hypothetical protein -
  SHEWBE_RS07655 (SHEWBE_1782) - 1648959..1649519 (+) 561 WP_005497537.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40972.94 Da        Isoelectric Point: 5.4319

>NTDB_id=1142119 SHEWBE_RS07625 WP_112352166.1 1644360..1645472(+) (pilU) [Shewanella benthica strain DB21MT-2]
MDVRPFLKTMIARKASDLFITAGFPPSAKIDGELTPLSETSFSAAQSLDFVESLMSDEQKKEFHQTRECNFAFALQDLGR
FRVSAFWQREASGCVMRRIETQIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNANSRGHILTIED
PIEFVHEHRQCIITQREVGIDTESFAAALKSSLRQAPDVILIGEIRTQETMEFALAFAETGHLCMATLHANNANQALDRI
MNLVPESKHQQLLFDLSLNLRGIVAQQLVPTMDGKGRRAAIEILINTPRISSLIAKNELHLLKETMAKSNEQGMQTFDQA
LLALYSEGEISYADALHHADSPNDLRLMIKLQSSEQTGSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1142119 SHEWBE_RS07625 WP_112352166.1 1644360..1645472(+) (pilU) [Shewanella benthica strain DB21MT-2]
ATGGATGTTCGTCCTTTCTTAAAAACGATGATAGCTCGCAAGGCTTCGGATCTCTTCATCACGGCGGGTTTCCCCCCGAG
TGCCAAAATTGATGGTGAGCTAACACCATTGAGCGAAACTTCATTCTCAGCGGCTCAGTCATTGGATTTTGTCGAGTCTT
TGATGTCTGATGAGCAGAAGAAAGAATTTCATCAGACTCGTGAGTGCAATTTCGCCTTTGCATTGCAGGATTTGGGGCGT
TTTCGTGTCAGTGCTTTTTGGCAGAGAGAAGCATCGGGTTGTGTCATGCGCCGTATCGAGACTCAGATCCCAGATGTCGA
TGATCTGAAACTTCCCCCAATCCTGAAAGATCTGGTGATGAGCAAACGTGGACTCATCATCATGGTCGGTGGAACGGGCA
CGGGTAAGTCGACTTCCTTGGCCGCTTTGGTTGGGTACCGTAATGCTAACTCTCGTGGTCATATTCTGACTATCGAAGAC
CCGATTGAATTTGTTCATGAGCACCGCCAGTGCATCATTACCCAGAGAGAAGTGGGTATAGATACCGAGTCATTCGCTGC
AGCGCTTAAAAGCTCACTGCGCCAGGCGCCAGATGTGATCTTAATCGGTGAAATTCGAACTCAAGAGACCATGGAATTCG
CCCTGGCGTTTGCCGAAACCGGCCACCTATGCATGGCGACCTTGCATGCGAACAATGCCAACCAGGCACTGGATCGTATC
ATGAACTTAGTGCCGGAGAGTAAGCATCAGCAGCTATTGTTCGACCTCTCGTTGAACTTAAGGGGAATCGTTGCTCAACA
GTTGGTGCCGACCATGGATGGCAAAGGCCGACGTGCGGCCATCGAAATTTTGATTAACACCCCACGTATCAGTAGTTTGA
TTGCTAAAAATGAGCTGCATTTACTCAAGGAGACCATGGCCAAGTCTAATGAGCAGGGGATGCAGACCTTCGATCAGGCC
TTGTTGGCTCTATATAGCGAGGGTGAGATCAGTTATGCCGATGCCTTACATCATGCTGACTCCCCTAATGATTTAAGATT
GATGATTAAGTTGCAGAGTTCGGAGCAAACGGGCTCAGGCTTTATGGAAGGGGTGACGCTAGATTTGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A330M0G8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.022

100

0.614
  pilU Acinetobacter baylyi ADP1

61.932

95.135

0.589
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.159

93.243

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362

Multiple sequence alignment