Detailed information NTG1134522

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: JMW95_RS03805
• Genome accession: NZ_LR890651
• Coordinates: 790623..791603 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: A0A979GH91
• Organism: Escherichia coli strain MINF_2E isolate MINF_2E
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 785623..796603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMW95_RS03775	yggM	786138..787145 (+)	1008	WP_000745217.1	DUF1202 family protein	-
JMW95_RS03780	hemW	787300..788436 (-)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
JMW95_RS03785	rdgB	788429..789022 (-)	594	WP_001174745.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
JMW95_RS03790	yggU	789030..789320 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
JMW95_RS03795	yggT	789317..789883 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
JMW95_RS03800	yggS	789901..790605 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
JMW95_RS03805	pilT	790623..791603 (+)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
JMW95_RS03810	ruvX	791779..792195 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
JMW95_RS03815	yqgE	792195..792758 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
JMW95_RS03820	gshB	792867..793817 (-)	951	WP_000593270.1	glutathione synthase	-
JMW95_RS03825	rsmE	793830..794561 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
JMW95_RS03830	endA	794641..795348 (-)	708	WP_000286500.1	deoxyribonuclease I	-
JMW95_RS03835	yggI	795443..795940 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=1134522 JMW95_RS03805 WP_001055620.1 790623..791603(+) (pilT) [Escherichia coli strain MINF_2E isolate MINF_2E]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1134522 JMW95_RS03805 WP_001055620.1 790623..791603(+) (pilT) [Escherichia coli strain MINF_2E isolate MINF_2E]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365