Detailed information NTG1134371

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: JMW69_RS04225
• Genome accession: NZ_LR890606
• Coordinates: 870635..871615 (+)
• Length: 326 a.a.
• NCBI ID: WP_001326494.1
• Uniprot ID: D3GUL4
• Organism: Escherichia coli strain MINF_8D isolate MINF_8D
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 865635..876615

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMW69_RS04195	yggM	866150..867157 (+)	1008	WP_000745210.1	DUF1202 family protein	-
JMW69_RS04200	hemW	867312..868448 (-)	1137	WP_000239943.1	radical SAM family heme chaperone HemW	-
JMW69_RS04205	rdgB	868441..869034 (-)	594	WP_001174777.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
JMW69_RS04210	yggU	869042..869332 (-)	291	WP_000994920.1	DUF167 family protein YggU	-
JMW69_RS04215	yggT	869329..869895 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
JMW69_RS04220	yggS	869913..870617 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
JMW69_RS04225	pilT	870635..871615 (+)	981	WP_001326494.1	type IV pilus twitching motility protein PilT	Machinery gene
JMW69_RS04230	ruvX	871799..872215 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
JMW69_RS04235	yqgE	872215..872778 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
JMW69_RS04240	gshB	872887..873837 (-)	951	WP_000593273.1	glutathione synthase	-
JMW69_RS04245	rsmE	873850..874581 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
JMW69_RS04250	endA	874661..875368 (-)	708	WP_000286500.1	deoxyribonuclease I	-
JMW69_RS04255	yggI	875463..875960 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=1134371 JMW69_RS04225 WP_001326494.1 870635..871615(+) (pilT) [Escherichia coli strain MINF_8D isolate MINF_8D]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1134371 JMW69_RS04225 WP_001326494.1 870635..871615(+) (pilT) [Escherichia coli strain MINF_8D isolate MINF_8D]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: D3GUL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362