Detailed information NTG1133609

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: JMV91_RS19395
• Genome accession: NZ_LR890326
• Coordinates: 3986642..3987622 (-)
• Length: 326 a.a.
• NCBI ID: WP_001326494.1
• Uniprot ID: D3GUL4
• Organism: Escherichia coli strain MINF_1A isolate MINF_1A
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3981642..3992622

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMV91_RS19365	yggI	3982297..3982794 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
JMV91_RS19370	endA	3982889..3983596 (+)	708	WP_000286489.1	deoxyribonuclease I	-
JMV91_RS19375	rsmE	3983676..3984407 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
JMV91_RS19380	gshB	3984420..3985370 (+)	951	WP_000593273.1	glutathione synthase	-
JMV91_RS19385	yqgE	3985479..3986042 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
JMV91_RS19390	ruvX	3986042..3986458 (+)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
JMV91_RS19395	pilT	3986642..3987622 (-)	981	WP_001326494.1	type IV pilus twitching motility protein PilT	Machinery gene
JMV91_RS19400	yggS	3987640..3988344 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
JMV91_RS19405	yggT	3988362..3988928 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
JMV91_RS19410	yggU	3988925..3989215 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
JMV91_RS19415	rdgB	3989223..3989816 (+)	594	WP_001174777.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
JMV91_RS19420	hemW	3989809..3990945 (+)	1137	WP_000239943.1	radical SAM family heme chaperone HemW	-
JMV91_RS19425	yggM	3991100..3992107 (-)	1008	WP_000745244.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=1133609 JMV91_RS19395 WP_001326494.1 3986642..3987622(-) (pilT) [Escherichia coli strain MINF_1A isolate MINF_1A]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1133609 JMV91_RS19395 WP_001326494.1 3986642..3987622(-) (pilT) [Escherichia coli strain MINF_1A isolate MINF_1A]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: D3GUL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362