Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLWA5_RS08240 Genome accession   NZ_LR877307
Coordinates   1932081..1933118 (+) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1927081..1938118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWA5_RS08210 (X12_001632) - 1927378..1927698 (-) 321 WP_080591330.1 hypothetical protein -
  ACLWA5_RS08215 (X12_001633) - 1927978..1928286 (-) 309 WP_016902350.1 DUF3861 family protein -
  ACLWA5_RS08220 (X12_001634) - 1928378..1928998 (-) 621 WP_026064287.1 M23 family metallopeptidase -
  ACLWA5_RS08225 (X12_001635) - 1929134..1929559 (-) 426 WP_026064288.1 HU family DNA-binding protein -
  ACLWA5_RS08230 (X12_001636) proC 1930159..1931004 (-) 846 WP_016902353.1 pyrroline-5-carboxylate reductase -
  ACLWA5_RS08235 (X12_001637) - 1931066..1931758 (-) 693 WP_016902354.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLWA5_RS08240 (X12_001638) pilT 1932081..1933118 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLWA5_RS08245 (X12_001639) pilU 1933241..1934371 (+) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLWA5_RS08250 (X12_001640) - 1934657..1935331 (+) 675 WP_016902356.1 YitT family protein -
  ACLWA5_RS08255 (X12_001641) - 1935458..1936003 (-) 546 WP_016902357.1 DNA-3-methyladenine glycosylase I -
  ACLWA5_RS08260 (X12_001642) - 1936328..1936894 (+) 567 WP_011051924.1 YqgE/AlgH family protein -
  ACLWA5_RS08265 (X12_001643) ruvX 1936887..1937354 (+) 468 WP_016902359.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=1132575 ACLWA5_RS08240 WP_003489919.1 1932081..1933118(+) (pilT) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1132575 ACLWA5_RS08240 WP_003489919.1 1932081..1933118(+) (pilT) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237]
ATGGATATCGCTGAACTATTGGCGTTTTCCGTCAAGAACAAGGCATCGGACCTGCATCTGTCGGCCGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCCCTGGACCACAAGCAGGTGCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCTTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGTTGATCGACCAGCCGCAGGGTTTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACGGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCATGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGATCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGTTGACTGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTATCCGAGTCGCTGCGCGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGACGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAAATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment