Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLWA5_RS08245 Genome accession   NZ_LR877307
Coordinates   1933241..1934371 (+) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1928241..1939371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWA5_RS08220 (X12_001634) - 1928378..1928998 (-) 621 WP_026064287.1 M23 family metallopeptidase -
  ACLWA5_RS08225 (X12_001635) - 1929134..1929559 (-) 426 WP_026064288.1 HU family DNA-binding protein -
  ACLWA5_RS08230 (X12_001636) proC 1930159..1931004 (-) 846 WP_016902353.1 pyrroline-5-carboxylate reductase -
  ACLWA5_RS08235 (X12_001637) - 1931066..1931758 (-) 693 WP_016902354.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLWA5_RS08240 (X12_001638) pilT 1932081..1933118 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLWA5_RS08245 (X12_001639) pilU 1933241..1934371 (+) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLWA5_RS08250 (X12_001640) - 1934657..1935331 (+) 675 WP_016902356.1 YitT family protein -
  ACLWA5_RS08255 (X12_001641) - 1935458..1936003 (-) 546 WP_016902357.1 DNA-3-methyladenine glycosylase I -
  ACLWA5_RS08260 (X12_001642) - 1936328..1936894 (+) 567 WP_011051924.1 YqgE/AlgH family protein -
  ACLWA5_RS08265 (X12_001643) ruvX 1936887..1937354 (+) 468 WP_016902359.1 Holliday junction resolvase RuvX -
  ACLWA5_RS08270 (X12_001644) - 1937370..1938317 (+) 948 WP_016902360.1 aspartate carbamoyltransferase catalytic subunit -
  ACLWA5_RS08275 (X12_001645) - 1938569..1939009 (+) 441 WP_016902361.1 OsmC family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1132576 ACLWA5_RS08245 WP_016902355.1 1933241..1934371(+) (pilU) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1132576 ACLWA5_RS08245 WP_016902355.1 1933241..1934371(+) (pilU) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 237]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACCCCGCTGACCGCGCAACAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTTGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTAGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATAATCACCCAGCGCGAAGTCGGCATCGATACTGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTGGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGTTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment