Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7A86_RS14140 Genome accession   NZ_LR861803
Coordinates   3368345..3369382 (-) Length   345 a.a.
NCBI ID   WP_104583089.1    Uniprot ID   -
Organism   Xanthomonas euroxanthea isolate     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3363345..3374382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7A86_RS14115 (XSP_002839) - 3363906..3364445 (+) 540 WP_119127834.1 DNA-3-methyladenine glycosylase I -
  H7A86_RS14120 (XSP_002840) - 3364442..3365164 (+) 723 WP_232099104.1 hypothetical protein -
  H7A86_RS14125 (XSP_002841) - 3365226..3366125 (+) 900 WP_119131210.1 DUF72 domain-containing protein -
  H7A86_RS14130 (XSP_002842) - 3366139..3366813 (-) 675 WP_164739766.1 YitT family protein -
  H7A86_RS14135 (XSP_002843) pilU 3367086..3368216 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7A86_RS14140 (XSP_002844) pilT 3368345..3369382 (-) 1038 WP_104583089.1 type IV pilus twitching motility protein PilT Machinery gene
  H7A86_RS14145 (XSP_002845) - 3369707..3370399 (+) 693 WP_119131209.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7A86_RS14150 (XSP_002846) proC 3370474..3371328 (+) 855 WP_104583087.1 pyrroline-5-carboxylate reductase -
  H7A86_RS14155 (XSP_002847) - 3371931..3372356 (+) 426 WP_119131208.1 HU family DNA-binding protein -
  H7A86_RS14160 (XSP_002848) - 3372532..3373110 (+) 579 WP_119131207.1 M23 family metallopeptidase -
  H7A86_RS14165 (XSP_002849) - 3373203..3373511 (+) 309 WP_104583084.1 DUF3861 family protein -
  H7A86_RS14170 (XSP_002850) - 3373566..3374108 (+) 543 WP_321186571.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38647.28 Da        Isoelectric Point: 6.6652

>NTDB_id=1132438 H7A86_RS14140 WP_104583089.1 3368345..3369382(-) (pilT) [Xanthomonas euroxanthea isolate]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEITSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1132438 H7A86_RS14140 WP_104583089.1 3368345..3369382(-) (pilT) [Xanthomonas euroxanthea isolate]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCATCTGTCGGCTGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCTCTGGACCACAAGCAGGTACATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCACGTCGCTGGCGCGC
TTCCGCGTCAATGCCTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGTTGGA
GGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGTTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
GTTGCTCAAGAAAGTGGGCGGCGGGCGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAATACGCCAAGGACAAGCGGATCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment