Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7A86_RS14135 Genome accession   NZ_LR861803
Coordinates   3367086..3368216 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas euroxanthea isolate     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3362086..3373216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7A86_RS14105 (XSP_002837) ruvX 3362534..3363001 (-) 468 WP_104583092.1 Holliday junction resolvase RuvX -
  H7A86_RS14110 (XSP_002838) - 3362994..3363560 (-) 567 WP_119131211.1 YqgE/AlgH family protein -
  H7A86_RS14115 (XSP_002839) - 3363906..3364445 (+) 540 WP_119127834.1 DNA-3-methyladenine glycosylase I -
  H7A86_RS14120 (XSP_002840) - 3364442..3365164 (+) 723 WP_232099104.1 hypothetical protein -
  H7A86_RS14125 (XSP_002841) - 3365226..3366125 (+) 900 WP_119131210.1 DUF72 domain-containing protein -
  H7A86_RS14130 (XSP_002842) - 3366139..3366813 (-) 675 WP_164739766.1 YitT family protein -
  H7A86_RS14135 (XSP_002843) pilU 3367086..3368216 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7A86_RS14140 (XSP_002844) pilT 3368345..3369382 (-) 1038 WP_104583089.1 type IV pilus twitching motility protein PilT Machinery gene
  H7A86_RS14145 (XSP_002845) - 3369707..3370399 (+) 693 WP_119131209.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7A86_RS14150 (XSP_002846) proC 3370474..3371328 (+) 855 WP_104583087.1 pyrroline-5-carboxylate reductase -
  H7A86_RS14155 (XSP_002847) - 3371931..3372356 (+) 426 WP_119131208.1 HU family DNA-binding protein -
  H7A86_RS14160 (XSP_002848) - 3372532..3373110 (+) 579 WP_119131207.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1132437 H7A86_RS14135 WP_016902355.1 3367086..3368216(-) (pilU) [Xanthomonas euroxanthea isolate]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1132437 H7A86_RS14135 WP_016902355.1 3367086..3368216(-) (pilU) [Xanthomonas euroxanthea isolate]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACGCCGCTGACCGCGCAACAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTGGAGGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGGATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGTCGCGGCCGGCGTGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGACGCCAAGACCCTGGCCCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment