Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KR100_RS00895 Genome accession   NZ_CP006269
Coordinates   171787..172863 (-) Length   358 a.a.
NCBI ID   WP_038542441.1    Uniprot ID   A0A076GXW3
Organism   Synechococcus sp. KORDI-100 strain KORDI 100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 166787..177863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KR100_RS00870 (KR100_00885) - 167144..167719 (+) 576 WP_038542431.1 RNA-binding protein -
  KR100_RS00875 (KR100_00890) dusA 167727..168731 (-) 1005 WP_038542433.1 tRNA dihydrouridine(20/20a) synthase DusA -
  KR100_RS00880 (KR100_00895) msrB 168801..169295 (+) 495 WP_038542435.1 peptide-methionine (R)-S-oxide reductase MsrB -
  KR100_RS00885 (KR100_00900) - 169267..170514 (+) 1248 WP_038542437.1 NAD(P)/FAD-dependent oxidoreductase -
  KR100_RS00890 (KR100_00905) - 170508..171767 (-) 1260 WP_038542439.1 type II secretion system F family protein -
  KR100_RS00895 (KR100_00910) pilT 171787..172863 (-) 1077 WP_038542441.1 type IV pilus twitching motility protein PilT Machinery gene
  KR100_RS00900 (KR100_00915) pilB/pilB1 172874..174595 (-) 1722 WP_204207798.1 GspE/PulE family protein Machinery gene
  KR100_RS00905 (KR100_00920) grpE 174833..175513 (+) 681 WP_038542444.1 nucleotide exchange factor GrpE -
  KR100_RS00910 (KR100_00925) dnaJ 175513..176640 (+) 1128 WP_038542447.1 molecular chaperone DnaJ -
  KR100_RS00915 (KR100_00930) - 176640..176867 (+) 228 WP_038542448.1 sulfurtransferase TusA family protein -
  KR100_RS00920 (KR100_00935) rsgA 176892..177773 (+) 882 WP_038547552.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39073.53 Da        Isoelectric Point: 5.8856

>NTDB_id=113064 KR100_RS00895 WP_038542441.1 171787..172863(-) (pilT) [Synechococcus sp. KORDI-100 strain KORDI 100]
MELMIEDLMEQLVGGGGSDLHLAAGQPPYGRFSGELRPMTEDPLTEEACNRLIFSMLNNGQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSNIPSAELLNLPPVVMETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYKSDKSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRHNPKPGQFGRVMAQEILINTAATANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVTAGDITRSEGLAKASKPGELERLISDD

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=113064 KR100_RS00895 WP_038542441.1 171787..172863(-) (pilT) [Synechococcus sp. KORDI-100 strain KORDI 100]
GTGGAGCTGATGATCGAAGATCTGATGGAGCAGCTTGTCGGAGGCGGCGGCAGCGATCTGCATCTCGCGGCGGGACAACC
CCCTTACGGACGCTTCAGCGGAGAACTCCGACCGATGACGGAGGATCCACTCACGGAAGAGGCCTGCAACCGGCTGATCT
TCTCGATGCTCAACAACGGTCAGCGCAAAACACTTGAGCAGACGTGGGAGCTGGATTGCGCCTACGGCCTGAAAGGGGTC
GCCCGTTTCCGCGTGAACGTCTACCGGCAGAAGGGCAGCTATGCCGCCTGCCTGAGAGCTCTGGGCAGCAATATTCCAAG
CGCCGAATTGCTGAATCTGCCCCCTGTGGTGATGGAGACCAGCAAACGACCCCGGGGACTCGTTCTGGTGACTGGACCGA
CCGGATCAGGCAAGACAACCACCCTGGCTGCACTGCTGGATCACATCAATCACACCCGTTCGGAACACATCCTCACCATC
GAGGATCCAATCGAATTCGTCTACAAGAGCGACAAAAGCCTGGTGCACCAGCGACAGCTGAACGAAGACACCCGCAGCTT
TGGAAACGCTCTGCGTGCCGCGTTGCGCGAAGACCCTGACGTGATTCTGGTGGGAGAAATGCGAGATCTCGAGACGATTC
AACTGGCGATCAGCGCTGCCGAGACAGGCCACCTGGTCTTCGGCACGTTGCACACCAGTTCCGCTGCTCAGACGGTCGAC
CGCATGGTGGATGTGTTTCCACCCGCCCAGCAAACCCAGATTCGAGTCCAGCTCTCGGGAAGTCTTGTCGCCGTCTTCTC
CCAGACACTTTGCCGACGTCACAACCCCAAACCGGGACAATTCGGCCGAGTGATGGCTCAGGAGATCCTGATCAACACCG
CCGCAACGGCGAACCTGATCCGTGAAGGCAAAACAGCGCAGCTCTACTCACAGATTCAGACCGGTGGAGAACTGGGCATG
CAGACCCTGGAAAAAGCCCTGGCCGATCTTGTGACGGCAGGGGACATCACTCGCTCGGAAGGGCTTGCCAAAGCCAGCAA
ACCCGGCGAACTGGAACGGCTGATCAGTGATGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A076GXW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.955

93.017

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.754

93.017

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.603

0.492
  pilT Acinetobacter baumannii strain A118

50.872

96.089

0.489
  pilT Acinetobacter nosocomialis M2

50.872

96.089

0.489
  pilT Acinetobacter baumannii D1279779

50.872

96.089

0.489
  pilT Legionella pneumophila strain ERS1305867

52.096

93.296

0.486
  pilT Legionella pneumophila strain Lp02

52.096

93.296

0.486
  pilT Acinetobacter baylyi ADP1

50.581

96.089

0.486
  pilT Neisseria meningitidis 8013

51.198

93.296

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilT Neisseria gonorrhoeae MS11

50.898

93.296

0.475
  pilU Pseudomonas stutzeri DSM 10701

39.031

98.045

0.383
  pilU Acinetobacter baylyi ADP1

37.644

97.207

0.366
  pilU Vibrio cholerae strain A1552

37.941

94.972

0.36

Multiple sequence alignment