Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L1Z78_RS25475 Genome accession   NZ_LR813084
Coordinates   5604712..5605755 (+) Length   347 a.a.
NCBI ID   WP_234639115.1    Uniprot ID   -
Organism   Delftia tsuruhatensis isolate BB1455     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5599712..5610755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1Z78_RS25455 (TML_05054) - 5600047..5601642 (-) 1596 WP_234639112.1 AMP-binding protein -
  L1Z78_RS25460 (TML_05055) - 5601803..5602510 (+) 708 WP_234639113.1 TetR/AcrR family transcriptional regulator -
  L1Z78_RS25465 (TML_05056) - 5602533..5603792 (+) 1260 WP_234639114.1 branched-chain amino acid ABC transporter substrate-binding protein -
  L1Z78_RS25470 (TML_05057) - 5603955..5604674 (-) 720 WP_234642266.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1Z78_RS25475 (TML_05058) pilT 5604712..5605755 (+) 1044 WP_234639115.1 type IV pilus twitching motility protein PilT Machinery gene
  L1Z78_RS25480 (TML_05059) - 5605787..5606419 (+) 633 WP_234639116.1 cyclic nucleotide-binding domain-containing protein -
  L1Z78_RS25485 (TML_05060) pilU 5606526..5607662 (+) 1137 WP_234639117.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1Z78_RS25490 (TML_05061) - 5607785..5608696 (+) 912 WP_234639118.1 NAD(P)-dependent oxidoreductase -
  L1Z78_RS25495 (TML_05062) - 5608767..5609498 (-) 732 WP_234639119.1 BON domain-containing protein -
  L1Z78_RS25500 (TML_05063) - 5609495..5610103 (-) 609 WP_234639120.1 SIS domain-containing protein -
  L1Z78_RS25505 (TML_05064) - 5610176..5610601 (-) 426 WP_234639121.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38337.08 Da        Isoelectric Point: 6.8353

>NTDB_id=1130393 L1Z78_RS25475 WP_234639115.1 5604712..5605755(+) (pilT) [Delftia tsuruhatensis isolate BB1455]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRIAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1130393 L1Z78_RS25475 WP_234639115.1 5604712..5605755(+) (pilT) [Delftia tsuruhatensis isolate BB1455]
GTGGACATCACCCAATTGCTGGCATTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCTGCGGGGCTGCCCCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAGACCGTCCACGCCATGGTCTACGACA
TCATGAGCGACTCCCAGCGCAAGGCCTACGAGGAGTTCCTGGAGGTGGACTTCTCGTTCGAGATCGAAGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGTACCGGGGCGCGGCCGCCGTCTTCCGGACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAATGCGCCCCGCATCTTCGCCGACCTGGCCCTGAAGCCCCGCGGACTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAGGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGATGACGCAGTCGTTCTCGGC
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGTGAAATGCGCGACCTGGAGACCATCCGCCTGG
CCATGACGGCCGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCTGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTCTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCATCGCCGCCCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCACAGATGTACTCCACCATCCAGACGAGTCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGATCTGGTTCGGCGCAACCTGATCAGCCCGGCCGAAGCCCGTGCCAAGGCCAAGATACCGGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment