Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1Z78_RS25485 Genome accession   NZ_LR813084
Coordinates   5606526..5607662 (+) Length   378 a.a.
NCBI ID   WP_234639117.1    Uniprot ID   -
Organism   Delftia tsuruhatensis isolate BB1455     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5601526..5612662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1Z78_RS25460 (TML_05055) - 5601803..5602510 (+) 708 WP_234639113.1 TetR/AcrR family transcriptional regulator -
  L1Z78_RS25465 (TML_05056) - 5602533..5603792 (+) 1260 WP_234639114.1 branched-chain amino acid ABC transporter substrate-binding protein -
  L1Z78_RS25470 (TML_05057) - 5603955..5604674 (-) 720 WP_234642266.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1Z78_RS25475 (TML_05058) pilT 5604712..5605755 (+) 1044 WP_234639115.1 type IV pilus twitching motility protein PilT Machinery gene
  L1Z78_RS25480 (TML_05059) - 5605787..5606419 (+) 633 WP_234639116.1 cyclic nucleotide-binding domain-containing protein -
  L1Z78_RS25485 (TML_05060) pilU 5606526..5607662 (+) 1137 WP_234639117.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1Z78_RS25490 (TML_05061) - 5607785..5608696 (+) 912 WP_234639118.1 NAD(P)-dependent oxidoreductase -
  L1Z78_RS25495 (TML_05062) - 5608767..5609498 (-) 732 WP_234639119.1 BON domain-containing protein -
  L1Z78_RS25500 (TML_05063) - 5609495..5610103 (-) 609 WP_234639120.1 SIS domain-containing protein -
  L1Z78_RS25505 (TML_05064) - 5610176..5610601 (-) 426 WP_234639121.1 YraN family protein -
  L1Z78_RS25510 (TML_05065) rsmI 5610601..5611536 (+) 936 WP_234639122.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41991.53 Da        Isoelectric Point: 6.9079

>NTDB_id=1130394 L1Z78_RS25485 WP_234639117.1 5606526..5607662(+) (pilU) [Delftia tsuruhatensis isolate BB1455]
MERDQATKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGRITKVSPQPLSPVHTLTLARSVMSDKQIADFERTKECNFAI
SPPGIGRFRVNAFIQQGRVGMVMRTIPLALPTIDSLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIVTVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRAQLLMDLSLNLRAMVSQRLLPKQDSKGRVAAIEVMLNSPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLGSQRAKSLDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1130394 L1Z78_RS25485 WP_234639117.1 5606526..5607662(+) (pilU) [Delftia tsuruhatensis isolate BB1455]
ATGGAAAGAGATCAGGCCACCAAGTTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACGGCGGAGTTCCCGCCCGCGATCAAGATCGACGGGCGCATCACCAAGGTGTCGCCGCAGCCGCTGTCGCCCGTGCACA
CGCTGACGCTGGCGCGCTCGGTCATGAGCGACAAGCAGATCGCCGACTTCGAGCGCACCAAGGAATGCAACTTCGCCATC
TCGCCCCCGGGCATCGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGATGCGGACGATTCC
GCTGGCCCTGCCCACCATCGACAGCCTGGGCGTGCCCCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAGTCCACCACGCTGGCGGCCATGGTGGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTGACCGTGGAGGATCCCATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGTGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCCGCGCTCAAGAACTCGCTGCGCCAGGCACCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCCATCGCCTTCTCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCAGAGGAACGTCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCCCAGCGCCTGCTGCCCAAGCAGGACAGCAAGGGCCGCGTGGCTGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAAAAAAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAATCCAACCTGATCACCTACGAGGACGCACTGCGCAACGCCGA
CTCGGTCAACGACCTGCGGTTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGTCGCTGGACCTGGCGGCAGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

56.425

94.709

0.534
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Acinetobacter baumannii D1279779

43.544

88.095

0.384
  pilT Acinetobacter baumannii strain A118

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

42.683

86.772

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.574

84.392

0.368
  pilT Vibrio cholerae strain A1552

43.574

84.392

0.368

Multiple sequence alignment