Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DENOEST_RS01200 Genome accession   NZ_LR778301
Coordinates   219294..220337 (+) Length   347 a.a.
NCBI ID   WP_145770096.1    Uniprot ID   A0A6S6XRS5
Organism   Denitratisoma oestradiolicum strain DSM 16959     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214294..225337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DENOEST_RS01170 (DENOEST_0247) - 215132..215890 (+) 759 WP_145770102.1 SDR family NAD(P)-dependent oxidoreductase -
  DENOEST_RS01175 (DENOEST_0248) - 215935..216693 (+) 759 WP_145770101.1 SDR family NAD(P)-dependent oxidoreductase -
  DENOEST_RS01180 (DENOEST_0249) - 216853..217170 (-) 318 WP_145770100.1 DUF167 domain-containing protein -
  DENOEST_RS01185 (DENOEST_0250) - 217167..217736 (-) 570 WP_145770099.1 YggT family protein -
  DENOEST_RS01190 (DENOEST_0251) proC 217743..218558 (-) 816 WP_145770098.1 pyrroline-5-carboxylate reductase -
  DENOEST_RS01195 (DENOEST_0252) - 218555..219250 (-) 696 WP_145770097.1 YggS family pyridoxal phosphate-dependent enzyme -
  DENOEST_RS01200 (DENOEST_0253) pilT 219294..220337 (+) 1044 WP_145770096.1 type IV pilus twitching motility protein PilT Machinery gene
  DENOEST_RS01205 (DENOEST_0255) pilU 220435..221571 (+) 1137 WP_145770095.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DENOEST_RS01210 (DENOEST_0256) - 221630..222460 (+) 831 WP_145770094.1 HDOD domain-containing protein -
  DENOEST_RS01215 (DENOEST_0258) - 222784..223374 (-) 591 WP_145770093.1 lysophospholipid acyltransferase family protein -
  DENOEST_RS01220 (DENOEST_0259) - 223384..223734 (-) 351 WP_145770092.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  DENOEST_RS01225 (DENOEST_0260) - 223934..224761 (-) 828 WP_145770091.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38165.68 Da        Isoelectric Point: 6.8883

>NTDB_id=1130252 DENOEST_RS01200 WP_145770096.1 219294..220337(+) (pilT) [Denitratisoma oestradiolicum strain DSM 16959]
MDITELLAFVVKNKASDLHLSSGLPPMIRVHGDIRRINLPPLEHKDVHAMVYDIMNDGQRKHYEENLECDFSFAIPGLAR
FRVNAYVQNRGAAAVLRTIPSTVLSLEQLNAPKSFKDIANQPRGIVLVTGPTGSGKSTTLAAMVDDINENEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQALLKTKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGGQFGMQTLDQN
LQDLVRRNIVSPSEARNKAANKDTFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1130252 DENOEST_RS01200 WP_145770096.1 219294..220337(+) (pilT) [Denitratisoma oestradiolicum strain DSM 16959]
ATGGACATCACCGAACTGCTGGCCTTCGTGGTCAAGAACAAGGCCTCCGACCTGCACCTCTCCTCCGGCCTGCCCCCCAT
GATCCGCGTCCATGGAGACATCCGGCGCATCAACCTGCCGCCCCTGGAACACAAGGACGTGCATGCGATGGTCTACGACA
TCATGAACGATGGTCAGCGCAAGCACTACGAGGAAAACCTGGAGTGCGACTTCTCCTTCGCCATTCCCGGCCTGGCCCGC
TTCCGGGTCAATGCCTATGTCCAGAACCGGGGGGCGGCGGCGGTGCTGCGGACCATTCCCTCCACGGTGTTGAGTCTGGA
GCAGCTCAATGCCCCCAAAAGCTTCAAGGATATCGCCAATCAGCCCCGGGGCATCGTGCTGGTCACCGGCCCCACCGGCT
CTGGCAAGTCCACCACGCTGGCGGCCATGGTGGACGACATCAACGAAAACGAATACGGCCACATTCTGACGGTGGAGGAC
CCGATCGAGTTCGTCCATGAATCGAAGAAATGCCTGATCAACCAGCGGGAGGTGGGACCCCACACCCTGTCCTTCAGCAA
CGCCTTGCGCTCGGCCTTGCGGGAAGATCCGGACGTGATCCTGGTGGGGGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCCTCTCGGCGGCGGAAACCGGCCACCTGGTCTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACGGTGGACCGGATT
GTGGACGTCTTCCCCGCCGCAGAAAAGGAAATGGTGCGATCCATGTTGTCGGAATCCCTGCGGGCGGTGATTTCCCAGGC
CCTGCTCAAGACCAAGGATGGCAGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACGCCGGCCATCCGCAACCTGA
TCCGGGAAAACAAGATCGCCCAGATGTATTCGGCGATCCAGACCGGCGGCCAGTTCGGCATGCAGACCCTGGACCAGAAC
CTGCAGGATCTGGTCCGACGCAACATCGTTTCCCCTTCCGAGGCCCGCAACAAGGCGGCCAACAAGGATACGTTCCCGGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S6XRS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.424

0.735
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria meningitidis 8013

72.174

99.424

0.718
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

71.884

99.424

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.406

0.651
  pilT Vibrio cholerae strain A1552

66.864

97.406

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Vibrio cholerae strain A1552

39.823

97.695

0.389

Multiple sequence alignment