Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DENOEST_RS01205 Genome accession   NZ_LR778301
Coordinates   220435..221571 (+) Length   378 a.a.
NCBI ID   WP_145770095.1    Uniprot ID   A0A6S6XTZ9
Organism   Denitratisoma oestradiolicum strain DSM 16959     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 215435..226571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DENOEST_RS01175 (DENOEST_0248) - 215935..216693 (+) 759 WP_145770101.1 SDR family NAD(P)-dependent oxidoreductase -
  DENOEST_RS01180 (DENOEST_0249) - 216853..217170 (-) 318 WP_145770100.1 DUF167 domain-containing protein -
  DENOEST_RS01185 (DENOEST_0250) - 217167..217736 (-) 570 WP_145770099.1 YggT family protein -
  DENOEST_RS01190 (DENOEST_0251) proC 217743..218558 (-) 816 WP_145770098.1 pyrroline-5-carboxylate reductase -
  DENOEST_RS01195 (DENOEST_0252) - 218555..219250 (-) 696 WP_145770097.1 YggS family pyridoxal phosphate-dependent enzyme -
  DENOEST_RS01200 (DENOEST_0253) pilT 219294..220337 (+) 1044 WP_145770096.1 type IV pilus twitching motility protein PilT Machinery gene
  DENOEST_RS01205 (DENOEST_0255) pilU 220435..221571 (+) 1137 WP_145770095.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DENOEST_RS01210 (DENOEST_0256) - 221630..222460 (+) 831 WP_145770094.1 HDOD domain-containing protein -
  DENOEST_RS01215 (DENOEST_0258) - 222784..223374 (-) 591 WP_145770093.1 lysophospholipid acyltransferase family protein -
  DENOEST_RS01220 (DENOEST_0259) - 223384..223734 (-) 351 WP_145770092.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  DENOEST_RS01225 (DENOEST_0260) - 223934..224761 (-) 828 WP_145770091.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -
  DENOEST_RS01230 (DENOEST_0261) - 224779..225783 (+) 1005 WP_145770090.1 glycosyltransferase family 9 protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42063.56 Da        Isoelectric Point: 6.4232

>NTDB_id=1130253 DENOEST_RS01205 WP_145770095.1 220435..221571(+) (pilU) [Denitratisoma oestradiolicum strain DSM 16959]
MERDEALKFMYQLLAMMVQKKGSDLFITAGFPPAIKIDGKMTPATTQPLTPQHTMELARSVMNDKQAAEFEATKECNFAI
SPANIGRFRVNAFVQQGRTGLVLRTINTSIPDFDELKLPLVLKDVAMTKRGLVLFVGGTGSGKSTSLAAMIGYRNQNSYG
HIITIEDPVEYVHEHRNCVITQREVGVDTDSWEAALKNTLRQAPDVILIGEIRDREVMEHAIAFAETGHLAMGTLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLKAVVSQRLIPLKEGKGRAAAVEILLNSPLISDLMGKGEVHGIKEIMKKSRELG
MQTFDQALFDLYEEGKISYEDALRFADSMNEVRLMIKLHGKEAKDKDPNAGIQNLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1130253 DENOEST_RS01205 WP_145770095.1 220435..221571(+) (pilU) [Denitratisoma oestradiolicum strain DSM 16959]
ATGGAACGCGACGAAGCACTCAAATTCATGTATCAGCTTCTTGCCATGATGGTCCAGAAGAAGGGCTCCGACCTGTTCAT
CACCGCCGGATTTCCGCCGGCCATCAAGATCGACGGCAAGATGACGCCGGCCACCACCCAGCCTTTGACCCCCCAACACA
CCATGGAACTGGCCCGTTCGGTGATGAACGACAAGCAGGCGGCGGAATTCGAGGCCACCAAGGAATGCAACTTTGCCATC
TCTCCGGCCAATATCGGCCGCTTCCGGGTCAATGCCTTCGTCCAGCAGGGGCGTACGGGCCTGGTGCTGCGAACCATCAA
CACCTCGATTCCCGATTTTGATGAACTCAAGCTGCCGCTGGTGCTGAAGGACGTGGCCATGACCAAGCGGGGCTTGGTGC
TGTTCGTCGGCGGCACCGGCTCGGGCAAGTCCACCTCACTGGCGGCCATGATCGGCTACCGCAACCAGAATTCCTACGGC
CACATCATCACCATCGAAGACCCGGTGGAATATGTTCATGAGCATCGCAACTGCGTCATCACCCAGCGGGAGGTGGGCGT
GGATACCGATTCCTGGGAGGCGGCCCTGAAGAACACCTTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAGATCCGCG
ACCGGGAGGTCATGGAGCACGCCATCGCCTTCGCCGAGACGGGCCACCTGGCCATGGGTACGCTCCATGCCAACAGCGCC
AACCAGGCCTTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGTTGCTGATGGACCTGTCCCTGAACCT
GAAGGCCGTGGTGTCCCAGCGCCTGATCCCTCTCAAGGAAGGCAAGGGCCGCGCTGCCGCGGTGGAAATCCTGCTCAACT
CCCCCCTGATTTCCGATCTGATGGGCAAGGGCGAAGTCCATGGCATCAAGGAAATTATGAAGAAGTCCCGGGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGATCTTTATGAAGAGGGCAAGATCAGCTACGAGGATGCCCTGCGCTTCGCCGA
CTCCATGAACGAGGTGCGGCTGATGATCAAGCTCCATGGCAAGGAGGCCAAGGATAAGGATCCCAATGCCGGCATCCAGA
ATCTGGACATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S6XTZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.706

91.799

0.603
  pilU Acinetobacter baylyi ADP1

61.095

91.799

0.561
  pilU Vibrio cholerae strain A1552

55.191

96.825

0.534
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.012

88.36

0.389
  pilT Acinetobacter baylyi ADP1

43.323

89.153

0.386
  pilT Pseudomonas aeruginosa PAK

43.807

87.566

0.384
  pilT Legionella pneumophila strain Lp02

43.807

87.566

0.384
  pilT Legionella pneumophila strain ERS1305867

43.807

87.566

0.384
  pilT Acinetobacter nosocomialis M2

42.9

87.566

0.376
  pilT Acinetobacter baumannii D1279779

42.9

87.566

0.376
  pilT Acinetobacter baumannii strain A118

42.9

87.566

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

87.302

0.368
  pilT Vibrio cholerae strain A1552

42.121

87.302

0.368
  pilT Pseudomonas stutzeri DSM 10701

41.441

88.095

0.365

Multiple sequence alignment