Detailed information NTG1130170

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: H1C97_RS14040
• Genome accession: NZ_LR778151
• Coordinates: 2908849..2909829 (-)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: A0A979GH91
• Organism: Escherichia coli isolate SC476
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2903849..2914829

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1C97_RS14010	yggI	2904512..2905009 (+)	498	WP_044687249.1	SprT family zinc-dependent metalloprotease	-
H1C97_RS14015	endA	2905104..2905811 (+)	708	WP_000286500.1	deoxyribonuclease I	-
H1C97_RS14020	rsmE	2905891..2906622 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
H1C97_RS14025	gshB	2906635..2907585 (+)	951	WP_000593273.1	glutathione synthase	-
H1C97_RS14030	yqgE	2907694..2908257 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
H1C97_RS14035	ruvX	2908257..2908673 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
H1C97_RS14040	pilT	2908849..2909829 (-)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
H1C97_RS14045	yggS	2909847..2910551 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
H1C97_RS14050	yggT	2910569..2911135 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
H1C97_RS14055	yggU	2911132..2911422 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
H1C97_RS14060	rdgB	2911430..2912023 (+)	594	WP_001174743.1	XTP/dITP diphosphatase	-
H1C97_RS14065	hemW	2912016..2913152 (+)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
H1C97_RS14070	-	2913460..2914446 (+)	987	WP_000784005.1	TRAP transporter substrate-binding protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=1130170 H1C97_RS14040 WP_001055620.1 2908849..2909829(-) (pilT) [Escherichia coli isolate SC476]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1130170 H1C97_RS14040 WP_001055620.1 2908849..2909829(-) (pilT) [Escherichia coli isolate SC476]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365