Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACL56_RS26060 Genome accession   NZ_LR743507
Coordinates   5501094..5502137 (+) Length   347 a.a.
NCBI ID   WP_339092687.1    Uniprot ID   -
Organism   Variovorax paradoxus isolate vvax     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5496094..5507137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACL56_RS26035 (VVAX_05189) - 5496552..5497277 (-) 726 WP_339092682.1 DUF2242 domain-containing protein -
  AACL56_RS26040 (VVAX_05190) - 5497362..5498015 (-) 654 WP_339092683.1 nucleoside/nucleotide kinase family protein -
  AACL56_RS26045 (VVAX_05191) - 5498049..5499323 (-) 1275 WP_339092684.1 Glu/Leu/Phe/Val dehydrogenase -
  AACL56_RS26050 (VVAX_05192) - 5499846..5500313 (+) 468 WP_339092685.1 Lrp/AsnC ligand binding domain-containing protein -
  AACL56_RS26055 (VVAX_05193) - 5500340..5501041 (-) 702 WP_339092686.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACL56_RS26060 (VVAX_05194) pilT 5501094..5502137 (+) 1044 WP_339092687.1 type IV pilus twitching motility protein PilT Machinery gene
  AACL56_RS26065 (VVAX_05195) pilU 5502202..5503338 (+) 1137 WP_339092688.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACL56_RS26070 (VVAX_05196) - 5503370..5504923 (-) 1554 WP_339092690.1 MFS transporter -
  AACL56_RS26075 (VVAX_05197) - 5504920..5505402 (-) 483 WP_339092691.1 MerR family transcriptional regulator -
  AACL56_RS26080 (VVAX_05198) - 5505491..5506399 (+) 909 WP_339092692.1 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38161.83 Da        Isoelectric Point: 6.8354

>NTDB_id=1129799 AACL56_RS26060 WP_339092687.1 5501094..5502137(+) (pilT) [Variovorax paradoxus isolate vvax]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGQGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1129799 AACL56_RS26060 WP_339092687.1 5501094..5502137(+) (pilT) [Variovorax paradoxus isolate vvax]
GTGGACATCACCCAATTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTTTCCGCCGGTCTTCCGCCCAT
GATCCGCGTCAATGGCGACGTGCGCCGCATCAACGTCGATGCGCTCGATCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACCCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCACGCGGCGCGGCCGCGGTGTTCCGGACCATTCCATCGAAGATCCTGACGCTGGA
GCAGCTCAATGCGCCGAAGATTTTCGGCGATCTCGCGCTCAAGCCGCGCGGCCTCGTGCTGGTGACCGGTCCCACGGGTT
CGGGCAAGTCGACCACGCTGGCGGCAATGGTCAACTACCTCAACGAAAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCTATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGATGACGCTGTCGTTCTCCAA
CGCACTGCGCTCCGCGCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGTTGCGCGACCTCGAGACCATCCGCCTCG
CCATGACCGCCGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAGGAAATGATCCGCGCGATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCGAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCCAGGGCATGCAGACGCTCGACCAGAAC
CTGACGGAGCTGGTGCGCCGCAATACCATCTCGGCAGCCGAAGCCCGCGGCAAGGCGAAGATCCCCGAAAACTTCCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment