Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACL56_RS26065 Genome accession   NZ_LR743507
Coordinates   5502202..5503338 (+) Length   378 a.a.
NCBI ID   WP_339092688.1    Uniprot ID   A0A679J845
Organism   Variovorax paradoxus isolate vvax     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5497202..5508338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACL56_RS26040 (VVAX_05190) - 5497362..5498015 (-) 654 WP_339092683.1 nucleoside/nucleotide kinase family protein -
  AACL56_RS26045 (VVAX_05191) - 5498049..5499323 (-) 1275 WP_339092684.1 Glu/Leu/Phe/Val dehydrogenase -
  AACL56_RS26050 (VVAX_05192) - 5499846..5500313 (+) 468 WP_339092685.1 Lrp/AsnC ligand binding domain-containing protein -
  AACL56_RS26055 (VVAX_05193) - 5500340..5501041 (-) 702 WP_339092686.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACL56_RS26060 (VVAX_05194) pilT 5501094..5502137 (+) 1044 WP_339092687.1 type IV pilus twitching motility protein PilT Machinery gene
  AACL56_RS26065 (VVAX_05195) pilU 5502202..5503338 (+) 1137 WP_339092688.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACL56_RS26070 (VVAX_05196) - 5503370..5504923 (-) 1554 WP_339092690.1 MFS transporter -
  AACL56_RS26075 (VVAX_05197) - 5504920..5505402 (-) 483 WP_339092691.1 MerR family transcriptional regulator -
  AACL56_RS26080 (VVAX_05198) - 5505491..5506399 (+) 909 WP_339092692.1 NAD(P)-dependent oxidoreductase -
  AACL56_RS26085 (VVAX_05199) - 5506833..5507648 (-) 816 WP_339092693.1 BON domain-containing protein -
  AACL56_RS26090 (VVAX_05200) - 5507645..5508289 (-) 645 WP_339092694.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41785.87 Da        Isoelectric Point: 6.4193

>NTDB_id=1129800 AACL56_RS26065 WP_339092688.1 5502202..5503338(+) (pilU) [Variovorax paradoxus isolate vvax]
MERDQASQFINDLLKLMVSRSGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKEVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEVMLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1129800 AACL56_RS26065 WP_339092688.1 5502202..5503338(+) (pilU) [Variovorax paradoxus isolate vvax]
GTGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAGCGGCAGCGACCTGTTCAT
CACGGCCGACTTTCCGCCGGCCATCAAGGTCGACGGAAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGGGTCAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTCAAGGAAGTGGCCATGACCAAGCGCGGCCTCACCA
TCCTGGTAGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCCATGATCGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTGACGGTGGAAGACCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTCGCCGAGACCGGTCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAGGAACGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTGTCGCAGCGGCTGGTGCCCACCGAGGACGGCCAGGGCCGCGTGGCCGCCGTCGAGGTGATGCTGAACA
CGCCGCTGATCTCCGACCTCATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAAGGCCACTCGATCACCTTCGAGGACGCGATCCGCAACGCCGA
CTCGGCGAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGATCTGTCGGCGGGCACGGAGC
ACTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679J845

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter nosocomialis M2

44.201

84.392

0.373
  pilT Acinetobacter baumannii D1279779

44.201

84.392

0.373
  pilT Acinetobacter baumannii strain A118

44.201

84.392

0.373
  pilT Acinetobacter baylyi ADP1

42.724

85.45

0.365

Multiple sequence alignment