Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QOY30_RS16495 Genome accession   NZ_LR699166
Coordinates   3451160..3452203 (+) Length   347 a.a.
NCBI ID   WP_283745714.1    Uniprot ID   -
Organism   Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3446160..3457203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOY30_RS16465 (SIDCL21_3467) - 3447433..3447777 (-) 345 WP_349496692.1 HU family DNA-binding protein -
  QOY30_RS16470 (SIDCL21_3468) - 3447888..3448481 (-) 594 WP_283745709.1 SRPBCC family protein -
  QOY30_RS16475 (SIDCL21_3469) - 3448536..3448820 (-) 285 WP_283745710.1 DUF167 family protein -
  QOY30_RS16480 (SIDCL21_3470) - 3448817..3449383 (-) 567 WP_283745711.1 YggT family protein -
  QOY30_RS16485 (SIDCL21_3471) proC 3449383..3450195 (-) 813 WP_283745712.1 pyrroline-5-carboxylate reductase -
  QOY30_RS16490 (SIDCL21_3472) - 3450427..3451107 (-) 681 WP_283745713.1 YggS family pyridoxal phosphate-dependent enzyme -
  QOY30_RS16495 (SIDCL21_3473) pilT 3451160..3452203 (+) 1044 WP_283745714.1 type IV pilus twitching motility protein PilT Machinery gene
  QOY30_RS16500 (SIDCL21_3474) pilU 3452251..3453387 (+) 1137 WP_283745715.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QOY30_RS16505 (SIDCL21_3475) - 3453479..3453943 (-) 465 WP_283745716.1 DoxX family protein -
  QOY30_RS16510 (SIDCL21_3476) - 3453940..3454716 (-) 777 WP_283745717.1 DNA-binding domain-containing protein -
  QOY30_RS16515 (SIDCL21_3477) - 3454706..3455551 (-) 846 WP_283745718.1 DUF692 domain-containing protein -
  QOY30_RS16520 (SIDCL21_3478) - 3455551..3455814 (-) 264 WP_283745719.1 DUF2282 domain-containing protein -
  QOY30_RS16525 (SIDCL21_3479) msrA 3456008..3456634 (-) 627 WP_283745720.1 peptide-methionine (S)-S-oxide reductase MsrA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38349.28 Da        Isoelectric Point: 6.9316

>NTDB_id=1129330 QOY30_RS16495 WP_283745714.1 3451160..3452203(+) (pilT) [Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDMRRINLPAMDHKEVHAMVYDIMNDGQRKHYEENKEVDFSFEVPNLAR
FRVNAFIQNRGAGAVMRTIPSKILTLEDLKCPPIFKDISEFPRGMVLVTGPTGSGKSTTLAAMVNHINENEMGHILTVED
PIEFVHESKKSLVNQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGTGRVAAHEIMICTPAIRNLIREAKVPQMYSAIQTGGNIGMQTLDQC
LSDMVKRNLVASSEARGKAANKDLFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1129330 QOY30_RS16495 WP_283745714.1 3451160..3452203(+) (pilT) [Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21]
ATGGATATCACCGAACTGCTCGCCTTCGGCGTGAAGAACAAAGCTTCCGACCTGCACCTTTCCGCAGGTTTGCCACCGAT
GATCCGCGTACACGGCGATATGCGCCGCATCAACCTGCCTGCCATGGATCACAAGGAAGTCCACGCCATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAACACTACGAAGAAAACAAGGAAGTCGATTTTTCGTTCGAGGTGCCAAACCTGGCGCGT
TTCCGCGTCAACGCCTTCATCCAGAATCGCGGTGCCGGCGCGGTGATGCGTACCATTCCTTCCAAGATACTCACGCTGGA
AGACCTCAAATGTCCGCCGATCTTCAAGGATATTTCCGAATTTCCGCGCGGCATGGTGCTGGTGACCGGCCCGACCGGTT
CCGGCAAATCGACCACGCTGGCGGCGATGGTGAACCATATCAACGAGAACGAGATGGGCCACATCCTGACCGTGGAAGAC
CCGATCGAATTCGTGCACGAATCGAAAAAGTCGCTGGTCAACCAGCGCGAAGTCGGCCCGCATACGCTGTCGTTCAACAA
CGCGCTGCGTTCCGCATTGCGCGAAGACCCGGATGTGATCCTCGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTCG
CAATGACGGCCGCCGAAACCGGCCACCTGGTCTTCGGCACGCTGCACACAAGCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCCGCCGATGAAAAGGAAATGGTGCGCGCGATGCTGTCCGAATCTTTGCGTGCGGTGATCTCGCAGAC
CCTGCTCAAAACCAAGGACGGCACAGGCCGCGTTGCGGCACATGAGATCATGATCTGTACCCCGGCGATCCGCAATCTGA
TCCGCGAAGCAAAAGTGCCGCAGATGTATTCCGCCATCCAGACAGGGGGCAACATCGGCATGCAGACGCTGGACCAATGC
CTGAGCGACATGGTGAAACGCAACCTTGTGGCTTCGTCAGAAGCGCGCGGGAAAGCTGCAAACAAGGATCTTTTCCCGGC
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

75.221

97.695

0.735
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria meningitidis 8013

72.012

98.847

0.712
  pilT Neisseria gonorrhoeae MS11

71.72

98.847

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.16

97.406

0.654
  pilT Vibrio cholerae strain A1552

67.16

97.406

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398
  pilU Acinetobacter baylyi ADP1

41.071

96.83

0.398

Multiple sequence alignment