Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QOY30_RS16500 Genome accession   NZ_LR699166
Coordinates   3452251..3453387 (+) Length   378 a.a.
NCBI ID   WP_283745715.1    Uniprot ID   -
Organism   Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3447251..3458387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOY30_RS16465 (SIDCL21_3467) - 3447433..3447777 (-) 345 WP_349496692.1 HU family DNA-binding protein -
  QOY30_RS16470 (SIDCL21_3468) - 3447888..3448481 (-) 594 WP_283745709.1 SRPBCC family protein -
  QOY30_RS16475 (SIDCL21_3469) - 3448536..3448820 (-) 285 WP_283745710.1 DUF167 family protein -
  QOY30_RS16480 (SIDCL21_3470) - 3448817..3449383 (-) 567 WP_283745711.1 YggT family protein -
  QOY30_RS16485 (SIDCL21_3471) proC 3449383..3450195 (-) 813 WP_283745712.1 pyrroline-5-carboxylate reductase -
  QOY30_RS16490 (SIDCL21_3472) - 3450427..3451107 (-) 681 WP_283745713.1 YggS family pyridoxal phosphate-dependent enzyme -
  QOY30_RS16495 (SIDCL21_3473) pilT 3451160..3452203 (+) 1044 WP_283745714.1 type IV pilus twitching motility protein PilT Machinery gene
  QOY30_RS16500 (SIDCL21_3474) pilU 3452251..3453387 (+) 1137 WP_283745715.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QOY30_RS16505 (SIDCL21_3475) - 3453479..3453943 (-) 465 WP_283745716.1 DoxX family protein -
  QOY30_RS16510 (SIDCL21_3476) - 3453940..3454716 (-) 777 WP_283745717.1 DNA-binding domain-containing protein -
  QOY30_RS16515 (SIDCL21_3477) - 3454706..3455551 (-) 846 WP_283745718.1 DUF692 domain-containing protein -
  QOY30_RS16520 (SIDCL21_3478) - 3455551..3455814 (-) 264 WP_283745719.1 DUF2282 domain-containing protein -
  QOY30_RS16525 (SIDCL21_3479) msrA 3456008..3456634 (-) 627 WP_283745720.1 peptide-methionine (S)-S-oxide reductase MsrA -
  QOY30_RS16530 (SIDCL21_3480) - 3456653..3458350 (-) 1698 WP_283745721.1 cytochrome c biogenesis protein DipZ -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42180.37 Da        Isoelectric Point: 6.7403

>NTDB_id=1129331 QOY30_RS16500 WP_283745715.1 3452251..3453387(+) (pilU) [Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21]
MERDQATELVHNLLRGMVSQKASDLFITSGFPPAFKVDGKMTPVSSQALTSQHTQELARSIMNDRQAAEFESTHECNFAI
SPPGIGRFRVNVFMQQQRVGMVLRTITTQIPTFQELGLPDVLKDVVMTKRGLVILVGGTGSGKSSSLAAMLGHRNHNSYG
HIITIEDPVEFVHDHDKCIVTHREVGVDTDNWHAALKNTLRQAPDVILIGEIRDRETMEYAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERREQLLMDLSLNIKALISQRLIPKRDSKGRAAAFEILLNSPLIADLIFKGNVHEIKEVMSKSRELG
MQTFDQALFILHEAGAISYEEALKNADSVNDLRLKIKLEGRATKDRDVMSGLDNLRMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1129331 QOY30_RS16500 WP_283745715.1 3452251..3453387(+) (pilU) [Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21]
ATGGAAAGAGACCAGGCTACCGAGCTAGTTCACAATCTTTTGCGCGGGATGGTCTCGCAAAAGGCATCCGACCTGTTCAT
CACTTCCGGCTTCCCGCCCGCATTCAAGGTAGACGGCAAGATGACGCCCGTTTCCAGCCAGGCGCTGACGTCGCAGCACA
CCCAGGAACTGGCGCGCAGCATCATGAACGACCGCCAGGCGGCCGAATTTGAATCAACCCACGAGTGCAATTTCGCCATC
AGCCCACCCGGCATCGGGCGCTTCCGCGTCAATGTGTTCATGCAGCAGCAACGCGTGGGCATGGTGCTGCGTACCATCAC
CACCCAAATTCCCACTTTTCAGGAGCTGGGACTGCCGGACGTCCTGAAGGATGTGGTGATGACCAAGCGCGGCCTGGTGA
TCCTGGTCGGCGGAACCGGATCCGGTAAATCCTCTTCGCTGGCTGCCATGCTCGGTCATCGCAACCACAACAGTTACGGC
CACATCATCACCATCGAGGACCCGGTTGAGTTTGTGCATGACCACGACAAGTGCATCGTCACCCATCGCGAAGTGGGCGT
AGATACCGACAACTGGCACGCCGCGCTGAAAAATACTTTGCGCCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGCG
ACCGCGAGACCATGGAATATGCGGTGGCTTTCGCCGAAACCGGCCACTTGTGCATGGCCACACTGCACGCCAACAGCGCC
AACCAGGCACTCGACCGCATCATCAACTTCTTCCCGGAAGAACGGCGTGAACAATTGCTGATGGACTTGTCGCTCAACAT
CAAGGCGCTCATTTCGCAACGCCTGATCCCGAAGCGCGACAGCAAGGGCCGCGCTGCCGCCTTCGAGATCCTGCTCAACT
CACCACTAATCGCCGACCTCATTTTCAAGGGCAATGTGCACGAGATCAAGGAAGTCATGTCCAAGTCGCGTGAACTCGGC
ATGCAAACCTTCGATCAGGCACTCTTCATACTGCACGAGGCGGGCGCTATCAGCTACGAGGAAGCGCTCAAGAACGCGGA
TTCCGTGAACGACCTGCGGCTGAAGATCAAACTGGAGGGCCGGGCCACCAAAGACAGGGACGTGATGAGCGGCCTGGATA
ATCTGCGGATGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.162

91.534

0.587
  pilU Acinetobacter baylyi ADP1

56.948

97.09

0.553
  pilU Vibrio cholerae strain A1552

56.609

92.063

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.478

89.683

0.381
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Acinetobacter nosocomialis M2

42.042

88.095

0.37
  pilT Acinetobacter baumannii D1279779

42.042

88.095

0.37
  pilT Acinetobacter baumannii strain A118

42.042

88.095

0.37

Multiple sequence alignment