Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5P1_RS18835 Genome accession   NZ_LR594689
Coordinates   3876906..3877949 (-) Length   347 a.a.
NCBI ID   WP_068674713.1    Uniprot ID   -
Organism   Variovorax sp. WDL1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3871906..3882949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P1_RS18810 (G3W93_RS18810) - 3872439..3872804 (+) 366 WP_068674903.1 YraN family protein -
  E5P1_RS18815 (G3W93_RS18815) - 3872898..3873524 (+) 627 WP_068674717.1 SIS domain-containing protein -
  E5P1_RS18820 (G3W93_RS18820) - 3873521..3874255 (+) 735 WP_102905120.1 BON domain-containing protein -
  E5P1_RS18825 (G3W93_RS18825) - 3874550..3875470 (-) 921 WP_068674716.1 NAD(P)-dependent oxidoreductase -
  E5P1_RS18830 (G3W93_RS18830) pilU 3875506..3876642 (-) 1137 WP_068674715.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5P1_RS18835 (G3W93_RS18835) pilT 3876906..3877949 (-) 1044 WP_068674713.1 type IV pilus twitching motility protein PilT Machinery gene
  E5P1_RS18840 (G3W93_RS18840) - 3877987..3878673 (+) 687 WP_102905122.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5P1_RS18845 (G3W93_RS18845) - 3878681..3879160 (-) 480 WP_068674711.1 Lrp/AsnC ligand binding domain-containing protein -
  E5P1_RS18850 (G3W93_RS18850) - 3879256..3882219 (+) 2964 WP_068674709.1 L-glutamate gamma-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38139.86 Da        Isoelectric Point: 6.8845

>NTDB_id=1128272 E5P1_RS18835 WP_068674713.1 3876906..3877949(-) (pilT) [Variovorax sp. WDL1]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPRIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVVSQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREGKVAQMYSSIQTGSGQGMQTLDQC
LTDLVRRNVISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1128272 E5P1_RS18835 WP_068674713.1 3876906..3877949(-) (pilT) [Variovorax sp. WDL1]
GTGGACATCACCCAACTGCTGGCCTTCAGCGTGAAGAACAAGGCCTCGGACTTGCATCTGTCATCCGGCCTGCCACCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACGTCGACGCGCTCGACCACAAGGCCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAATTCCTCGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCCCGCGGCGCGGCCGCGGTGTTCCGCACCATTCCCTCGAAGATCCTCACACTGGA
GCAGCTCAACGCGCCCAGGATTTTCGGTGAACTCGCGCTCAAGCCCCGTGGCCTGGTGCTGGTGACCGGCCCGACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCGATGATCAACTACCTCAACGAGACCGAATACGGCCACATCCTGACGGTGGAGGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAACGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCAA
CGCCCTGCGCTCGGCGCTGCGCGAGGACCCCGACGCGATCCTGGTCGGCGAAATGCGCGACCTGGAAACGATCCGCCTGG
CGATGACTGCCGCCGAGACAGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCGGCCGCCAAGACGATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAGAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGGTCTCGCAGAC
CCTGTGCAAGACCAAGGACGGCCAGGGACGGGTGGCGGCGCACGAGATCATGCTGGGCACCTCCGCCATCCGCAACCTGA
TCCGCGAGGGCAAGGTGGCGCAGATGTACTCCTCGATCCAGACCGGCAGCGGCCAGGGCATGCAGACGCTGGACCAGTGC
CTGACGGACCTCGTGCGGCGCAATGTCATCTCCGCGGCAGAAGCCCGCGGCAAGGCCAAGATCCCCGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.072

99.424

0.746
  pilT Acinetobacter baumannii D1279779

75.072

99.424

0.746
  pilT Acinetobacter baumannii strain A118

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment