Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5P1_RS18830 Genome accession   NZ_LR594689
Coordinates   3875506..3876642 (-) Length   378 a.a.
NCBI ID   WP_068674715.1    Uniprot ID   A0A2J7W6R2
Organism   Variovorax sp. WDL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3870506..3881642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P1_RS18800 (G3W93_RS18800) - 3870709..3871437 (+) 729 WP_068674905.1 glutathione S-transferase -
  E5P1_RS18805 (G3W93_RS18805) rsmI 3871479..3872414 (-) 936 WP_068674718.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  E5P1_RS18810 (G3W93_RS18810) - 3872439..3872804 (+) 366 WP_068674903.1 YraN family protein -
  E5P1_RS18815 (G3W93_RS18815) - 3872898..3873524 (+) 627 WP_068674717.1 SIS domain-containing protein -
  E5P1_RS18820 (G3W93_RS18820) - 3873521..3874255 (+) 735 WP_102905120.1 BON domain-containing protein -
  E5P1_RS18825 (G3W93_RS18825) - 3874550..3875470 (-) 921 WP_068674716.1 NAD(P)-dependent oxidoreductase -
  E5P1_RS18830 (G3W93_RS18830) pilU 3875506..3876642 (-) 1137 WP_068674715.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5P1_RS18835 (G3W93_RS18835) pilT 3876906..3877949 (-) 1044 WP_068674713.1 type IV pilus twitching motility protein PilT Machinery gene
  E5P1_RS18840 (G3W93_RS18840) - 3877987..3878673 (+) 687 WP_102905122.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5P1_RS18845 (G3W93_RS18845) - 3878681..3879160 (-) 480 WP_068674711.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42062.31 Da        Isoelectric Point: 6.5042

>NTDB_id=1128271 E5P1_RS18830 WP_068674715.1 3875506..3876642(-) (pilU) [Variovorax sp. WDL1]
MERDQASQFINELLKLMVSRNGSDLFITADFPPAIKIDGKVTKVSQQALGSQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVTMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLRSLISQRLIPTEDGRGRIAAVEILLNTPLITDLIYKGEVGEIKEVMKKSRNLG
MQTFDQALFDLFETNMISFEDALRNADSANDLRLQIKLNSQRARTTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1128271 E5P1_RS18830 WP_068674715.1 3875506..3876642(-) (pilU) [Variovorax sp. WDL1]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGAACTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCCGCGATCAAGATCGATGGGAAGGTCACCAAGGTGTCGCAGCAGGCCTTGGGCTCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
GGCCAAGCTTCCCACCATCGACGGGCTGGGCATGCCGCAGGTGCTGAAGGACGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAATTCCTTCGGC
CACATCGTGACGGTGGAAGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCAT
CGACACCGACAGCTGGGAGGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGAACATGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCTCGCTGATCTCGCAGCGCCTGATCCCGACCGAGGACGGCCGCGGCCGCATCGCCGCGGTGGAGATCCTGCTGAACA
CGCCGCTGATCACCGACCTGATCTACAAGGGCGAGGTGGGCGAGATCAAGGAGGTCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGACCAACATGATTTCCTTCGAGGACGCACTGCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCACGACCGATCTGTCGGCCGGGACAGAGC
ACTTCGCGATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J7W6R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.857

92.593

0.582
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

42.478

89.683

0.381
  pilT Legionella pneumophila strain Lp02

43.769

87.037

0.381
  pilT Legionella pneumophila strain ERS1305867

43.769

87.037

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365

Multiple sequence alignment