Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5CHR_RS02640 Genome accession   NZ_LR594675
Coordinates   558910..559953 (-) Length   347 a.a.
NCBI ID   WP_162578250.1    Uniprot ID   A0A6P2DV11
Organism   Variovorax sp. PBS-H4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 553910..564953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5CHR_RS02615 (H4CHR_00522) - 554654..555019 (+) 366 WP_162578245.1 YraN family protein -
  E5CHR_RS02620 (H4CHR_00523) - 555114..555743 (+) 630 WP_162578246.1 SIS domain-containing protein -
  E5CHR_RS02625 (H4CHR_00524) - 555740..556483 (+) 744 WP_162578247.1 BON domain-containing protein -
  E5CHR_RS02630 (H4CHR_00525) - 556553..557473 (-) 921 WP_162578248.1 NAD(P)-dependent oxidoreductase -
  E5CHR_RS02635 (H4CHR_00526) pilU 557510..558646 (-) 1137 WP_162578249.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5CHR_RS02640 (H4CHR_00527) pilT 558910..559953 (-) 1044 WP_162578250.1 type IV pilus twitching motility protein PilT Machinery gene
  E5CHR_RS02645 (H4CHR_00528) - 559992..560678 (+) 687 WP_162578251.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5CHR_RS02650 (H4CHR_00529) - 560684..561163 (-) 480 WP_162578252.1 Lrp/AsnC ligand binding domain-containing protein -
  E5CHR_RS02655 (H4CHR_00530) - 561388..562662 (+) 1275 WP_162578253.1 Glu/Leu/Phe/Val dehydrogenase -
  E5CHR_RS02660 (H4CHR_00531) - 562773..563396 (+) 624 WP_162578254.1 DUF2242 domain-containing protein -
  E5CHR_RS02665 (H4CHR_00532) - 563403..563630 (-) 228 WP_162578255.1 hypothetical protein -
  E5CHR_RS02670 (H4CHR_00533) - 563836..564213 (+) 378 WP_162578256.1 VOC family protein -
  E5CHR_RS02675 (H4CHR_00534) - 564356..564553 (+) 198 WP_162578257.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38097.82 Da        Isoelectric Point: 6.8844

>NTDB_id=1128242 E5CHR_RS02640 WP_162578250.1 558910..559953(-) (pilT) [Variovorax sp. PBS-H4]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMINYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVVSQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREGKVAQMYSSIQTGSGQGMQTLDQC
LTDLVRRNVISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1128242 E5CHR_RS02640 WP_162578250.1 558910..559953(-) (pilT) [Variovorax sp. PBS-H4]
GTGGACATCACCCAACTGCTGGCCTTCAGCGTAAAGAACAAGGCCTCGGACTTGCATCTGTCATCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACGTCGACGCGCTCGACCACAAGGCCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAATTCCTCGAGGTCGACTTTTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCCCGCGGCGCGGCGGCGGTGTTCCGGACCATCCCCTCGAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATTTTCGGCGACCTCGCGCTCAAGCCCCGGGGCCTGGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGATCAACTACCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAAGAC
CCGATCGAGTTCGTCCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCAA
CGCCCTGCGCTCGGCGCTGCGCGAGGACCCCGACGCGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTCG
CGATGACCGCCGCCGAGACGGGCCACCTGGTGTTCGGCACTCTGCACACCTCCTCGGCCGCCAAGACGATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGGTCTCGCAGAC
TTTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACCTCGGCCATCCGCAACCTGA
TCCGCGAGGGCAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCAGCGGCCAGGGCATGCAGACGCTGGACCAGTGC
CTGACGGACCTGGTGCGGCGCAATGTCATTTCCGCCGCCGAGGCACGCGGCAAGGCCAAGATCCCTGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2DV11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment