Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5CHR_RS02635 Genome accession   NZ_LR594675
Coordinates   557510..558646 (-) Length   378 a.a.
NCBI ID   WP_162578249.1    Uniprot ID   -
Organism   Variovorax sp. PBS-H4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552510..563646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5CHR_RS02605 (H4CHR_00520) - 552796..553677 (+) 882 WP_162578243.1 hypothetical protein -
  E5CHR_RS02610 (H4CHR_00521) rsmI 553697..554629 (-) 933 WP_174255682.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  E5CHR_RS02615 (H4CHR_00522) - 554654..555019 (+) 366 WP_162578245.1 YraN family protein -
  E5CHR_RS02620 (H4CHR_00523) - 555114..555743 (+) 630 WP_162578246.1 SIS domain-containing protein -
  E5CHR_RS02625 (H4CHR_00524) - 555740..556483 (+) 744 WP_162578247.1 BON domain-containing protein -
  E5CHR_RS02630 (H4CHR_00525) - 556553..557473 (-) 921 WP_162578248.1 NAD(P)-dependent oxidoreductase -
  E5CHR_RS02635 (H4CHR_00526) pilU 557510..558646 (-) 1137 WP_162578249.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5CHR_RS02640 (H4CHR_00527) pilT 558910..559953 (-) 1044 WP_162578250.1 type IV pilus twitching motility protein PilT Machinery gene
  E5CHR_RS02645 (H4CHR_00528) - 559992..560678 (+) 687 WP_162578251.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5CHR_RS02650 (H4CHR_00529) - 560684..561163 (-) 480 WP_162578252.1 Lrp/AsnC ligand binding domain-containing protein -
  E5CHR_RS02655 (H4CHR_00530) - 561388..562662 (+) 1275 WP_162578253.1 Glu/Leu/Phe/Val dehydrogenase -
  E5CHR_RS02660 (H4CHR_00531) - 562773..563396 (+) 624 WP_162578254.1 DUF2242 domain-containing protein -
  E5CHR_RS02665 (H4CHR_00532) - 563403..563630 (-) 228 WP_162578255.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42102.33 Da        Isoelectric Point: 6.5901

>NTDB_id=1128241 E5CHR_RS02635 WP_162578249.1 557510..558646(-) (pilU) [Variovorax sp. PBS-H4]
MERDQASQFINELLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLNMPQVLKDVTMTKRGLCILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLIPTEDGRGRIAAVEILLNTPLITDLIYKGEVGEIKEVMKKSRNLG
MQTFDQALFDLFETHMISFEDALRNADSANDLRLQIKLNSQRARTTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1128241 E5CHR_RS02635 WP_162578249.1 557510..558646(-) (pilU) [Variovorax sp. PBS-H4]
ATGGAACGCGATCAAGCCAGTCAGTTCATCAACGAACTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGACTTCCCGCCGGCCATCAAGGTCGACGGGAAGGTCACCAAGGTGTCGCAGCAAGCCTTGGGCGCGCAGCACA
CGCTGGCACTCACGCGGTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
GGCCAAGCTTCCCACGATCGACGGGCTGAACATGCCGCAGGTGCTGAAGGACGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACCGGCTCAGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAATTCCTACGGC
CACATCGTGACGGTTGAAGACCCGGTGGAGTTCGTGCACCCGCACAAGAATTGCGTCGTGACCCAGCGCGAGGTCGGGAT
CGATACCGACAGCTGGGAGGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTCATTCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCGGTGGCCTTTGCCGAGACCGGGCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCGCGCAGCTGTTGATGGACCTGTCGCTCAACTT
GCGCTCGTTGATCTCGCAGCGCCTCATCCCCACCGAGGATGGCCGCGGCCGGATCGCCGCGGTGGAGATCCTGCTGAATA
CGCCGCTCATCACCGACCTGATCTACAAGGGCGAGGTCGGCGAGATCAAGGAGGTCATGAAGAAGAGCCGCAATCTGGGC
ATGCAGACCTTCGACCAGGCGTTGTTCGACCTGTTCGAGACCCACATGATTTCCTTCGAGGATGCGCTGCGCAACGCGGA
CTCCGCCAACGACCTTCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCACCACCGACCTGTCCGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.143

92.593

0.585
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Pseudomonas aeruginosa PAK

45.697

89.153

0.407
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

43.769

87.037

0.381
  pilT Legionella pneumophila strain ERS1305867

43.769

87.037

0.381
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment