Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WDLP6_RS02180 Genome accession   NZ_LR594671
Coordinates   444414..445457 (-) Length   347 a.a.
NCBI ID   WP_162591023.1    Uniprot ID   -
Organism   Variovorax sp. PBL-E5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439414..450457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDLP6_RS02155 (E5CHR_00431) - 440263..440625 (+) 363 WP_162594938.1 YraN family protein -
  WDLP6_RS02160 (E5CHR_00432) - 440711..441325 (+) 615 WP_162565571.1 SIS domain-containing protein -
  WDLP6_RS02165 (E5CHR_00433) - 441322..442059 (+) 738 WP_162591020.1 BON domain-containing protein -
  WDLP6_RS02170 (E5CHR_00434) - 442167..443084 (-) 918 WP_162591021.1 NAD(P)-dependent oxidoreductase -
  WDLP6_RS02175 (E5CHR_00435) pilU 443125..444261 (-) 1137 WP_162591022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDLP6_RS02180 (E5CHR_00436) pilT 444414..445457 (-) 1044 WP_162591023.1 type IV pilus twitching motility protein PilT Machinery gene
  WDLP6_RS02185 (E5CHR_00437) - 445497..446183 (+) 687 WP_162591024.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDLP6_RS02190 (E5CHR_00438) - 446307..447578 (+) 1272 WP_162591025.1 Glu/Leu/Phe/Val dehydrogenase -
  WDLP6_RS02195 (E5CHR_00439) - 447698..448342 (+) 645 WP_162565578.1 DUF2242 domain-containing protein -
  WDLP6_RS02200 (E5CHR_00440) - 448351..448746 (-) 396 WP_162591026.1 response regulator -
  WDLP6_RS02205 (E5CHR_00441) - 448886..449119 (-) 234 WP_162591027.1 hypothetical protein -
  WDLP6_RS02210 (E5CHR_00442) rocF 449232..450143 (-) 912 WP_162591028.1 arginase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38120.84 Da        Isoelectric Point: 6.8886

>NTDB_id=1128221 WDLP6_RS02180 WP_162591023.1 444414..445457(-) (pilT) [Variovorax sp. PBL-E5]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIEGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMINFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREGKVAQMYSSIQTGSGQGMQTLDQN
LTDLVRRNVISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1128221 WDLP6_RS02180 WP_162591023.1 444414..445457(-) (pilT) [Variovorax sp. PBL-E5]
GTGGACATCACCCAACTGCTGGCCTTCAGCGTCAAGAACAAGGCGTCGGATCTGCATCTGTCGTCCGGGCTCCCGCCCAT
GATCCGGGTGCACGGCGACGTGCGGCGCATCAATGTCGACGCGCTCGACCACAAGGCCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAGGAATTCCTCGAAGTCGATTTCTCCTTCGAGATCGAGGGGCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATTCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTCGCGCTCAAGCCGCGCGGTCTCGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGATCAACTTCCTCAACGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACACTGTCCTTCGCGAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTCG
CGATGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACCTCCGCGATCCGCAACCTGA
TCCGCGAAGGCAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGCAGCGGCCAGGGCATGCAGACGCTGGACCAGAAC
CTCACCGATCTCGTGCGCCGCAACGTGATTTCGGCCGCGGAAGCGCGCGGCAAGGCCAAGATCCCCGAGAATTTCCCTGG
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment