Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WDLP6_RS02175 Genome accession   NZ_LR594671
Coordinates   443125..444261 (-) Length   378 a.a.
NCBI ID   WP_162591022.1    Uniprot ID   A0A6P2E2G8
Organism   Variovorax sp. PBL-E5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438125..449261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDLP6_RS02145 (E5CHR_00429) - 438554..439282 (+) 729 WP_162591019.1 glutathione S-transferase -
  WDLP6_RS02150 (E5CHR_00430) rsmI 439290..440228 (-) 939 WP_162565570.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  WDLP6_RS02155 (E5CHR_00431) - 440263..440625 (+) 363 WP_162594938.1 YraN family protein -
  WDLP6_RS02160 (E5CHR_00432) - 440711..441325 (+) 615 WP_162565571.1 SIS domain-containing protein -
  WDLP6_RS02165 (E5CHR_00433) - 441322..442059 (+) 738 WP_162591020.1 BON domain-containing protein -
  WDLP6_RS02170 (E5CHR_00434) - 442167..443084 (-) 918 WP_162591021.1 NAD(P)-dependent oxidoreductase -
  WDLP6_RS02175 (E5CHR_00435) pilU 443125..444261 (-) 1137 WP_162591022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDLP6_RS02180 (E5CHR_00436) pilT 444414..445457 (-) 1044 WP_162591023.1 type IV pilus twitching motility protein PilT Machinery gene
  WDLP6_RS02185 (E5CHR_00437) - 445497..446183 (+) 687 WP_162591024.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDLP6_RS02190 (E5CHR_00438) - 446307..447578 (+) 1272 WP_162591025.1 Glu/Leu/Phe/Val dehydrogenase -
  WDLP6_RS02195 (E5CHR_00439) - 447698..448342 (+) 645 WP_162565578.1 DUF2242 domain-containing protein -
  WDLP6_RS02200 (E5CHR_00440) - 448351..448746 (-) 396 WP_162591026.1 response regulator -
  WDLP6_RS02205 (E5CHR_00441) - 448886..449119 (-) 234 WP_162591027.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41866.99 Da        Isoelectric Point: 6.4189

>NTDB_id=1128220 WDLP6_RS02175 WP_162591022.1 443125..444261(-) (pilU) [Variovorax sp. PBL-E5]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLIPTEDGHGRIAAVEILLNTPLISDMIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAITFEDAIRNADSANDLRLQIKLNSQRARTPDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1128220 WDLP6_RS02175 WP_162591022.1 443125..444261(-) (pilU) [Variovorax sp. PBL-E5]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGATCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCCGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCACAGCAGGCGCTCGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCCGTCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACCGGGATCGGGCGCTTCCGCGTCAATGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTACCGACCATCGATGGCCTCGGCATGCCGCAGGTGCTCAAGGATGTGTCGATGACCAAGCGCGGCCTGACGA
TCCTGGTGGGCGCGACCGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTGACGGTCGAGGATCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTCGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCGGTCGCCTTCGCCGAGACCGGGCATCTGTGCATGGCCACCCTGCATGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAATTTCTTCCCCGAGGAACGCCGGGCCCAGTTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATCTCGCAGCGCCTGATCCCGACCGAGGACGGGCATGGCCGCATCGCCGCGGTCGAAATCCTGCTCAACA
CGCCGCTGATCTCCGACATGATCTTCAAGGGTGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGG
ATGCAGACCTTCGATCAGGCGCTGTTCGACCTGTTCGAGGGCAACGCGATCACCTTCGAGGATGCGATCCGCAACGCGGA
CTCGGCCAACGACCTGCGACTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCACGCCCGATCTGTCGGCAGGCACGGAAC
ATTTCGCAATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2E2G8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter baumannii D1279779

43.544

88.095

0.384
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Acinetobacter baumannii strain A118

43.544

88.095

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Legionella pneumophila strain Lp02

43.161

87.037

0.376
  pilT Legionella pneumophila strain ERS1305867

43.161

87.037

0.376
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment