Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5P2_RS02405 Genome accession   NZ_LR594666
Coordinates   488393..489436 (-) Length   347 a.a.
NCBI ID   WP_162565575.1    Uniprot ID   -
Organism   Variovorax sp. SRS16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483393..494436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P2_RS02380 (G3W92_RS02380) - 484361..484723 (+) 363 WP_162570313.1 YraN family protein -
  E5P2_RS02385 (G3W92_RS02385) - 484809..485423 (+) 615 WP_162565571.1 SIS domain-containing protein -
  E5P2_RS02390 (G3W92_RS02390) - 485420..486157 (+) 738 WP_162565572.1 BON domain-containing protein -
  E5P2_RS02395 (G3W92_RS02395) - 486264..487181 (-) 918 WP_162565573.1 NAD(P)-dependent oxidoreductase -
  E5P2_RS02400 (G3W92_RS02400) pilU 487222..488358 (-) 1137 WP_162565574.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5P2_RS02405 (G3W92_RS02405) pilT 488393..489436 (-) 1044 WP_162565575.1 type IV pilus twitching motility protein PilT Machinery gene
  E5P2_RS02410 (G3W92_RS02410) - 489476..490162 (+) 687 WP_162565576.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5P2_RS02415 (G3W92_RS02415) - 490286..491557 (+) 1272 WP_162565577.1 Glu/Leu/Phe/Val dehydrogenase -
  E5P2_RS02420 (G3W92_RS02420) - 491689..492321 (+) 633 WP_232080555.1 DUF2242 domain-containing protein -
  E5P2_RS02425 (G3W92_RS02425) - 492379..492612 (-) 234 WP_162565579.1 hypothetical protein -
  E5P2_RS02430 (G3W92_RS02430) rocF 492725..493636 (-) 912 WP_162565580.1 arginase -
  E5P2_RS02435 (G3W92_RS02435) - 493872..494411 (+) 540 WP_162565581.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38132.85 Da        Isoelectric Point: 6.8885

>NTDB_id=1128201 E5P2_RS02405 WP_162565575.1 488393..489436(-) (pilT) [Variovorax sp. SRS16]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMINFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREGKVAQMYSSIQTGSGQGMQTLDQN
LTDLVRRNVISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1128201 E5P2_RS02405 WP_162565575.1 488393..489436(-) (pilT) [Variovorax sp. SRS16]
GTGGACATCACCCAACTGCTGGCCTTCAGCGTCAAGAACAAGGCGTCGGATCTGCATCTGTCGTCCGGGCTCCCGCCCAT
GATCCGGGTGCACGGCGACGTGCGGCGCATCAATGTCGACGCGCTCGACCACAAGGCCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAGGAATTCCTCGAAGTCGATTTCTCCTTCGAGATCGACGGGCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATTCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTCGCGCTCAAGCCGCGCGGTCTCGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGATCAACTTCCTCAACGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACACTGTCCTTCGCGAA
CGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGACGCGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTCG
CGATGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACCTCCGCGATCCGCAACCTGA
TCCGCGAAGGCAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGCAGCGGCCAGGGCATGCAGACGCTGGACCAGAAC
CTCACCGATCTCGTGCGCCGCAACGTGATTTCGCCCGCGGAAGCGCGCGGCAAAGCAAAAATCCCCGAGAATTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment