Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5P2_RS02400 Genome accession   NZ_LR594666
Coordinates   487222..488358 (-) Length   378 a.a.
NCBI ID   WP_162565574.1    Uniprot ID   A0A8B6LF35
Organism   Variovorax sp. SRS16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482222..493358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P2_RS02370 (G3W92_RS02370) - 482652..483380 (+) 729 WP_162565569.1 glutathione S-transferase -
  E5P2_RS02375 (G3W92_RS02375) rsmI 483388..484326 (-) 939 WP_162565570.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  E5P2_RS02380 (G3W92_RS02380) - 484361..484723 (+) 363 WP_162570313.1 YraN family protein -
  E5P2_RS02385 (G3W92_RS02385) - 484809..485423 (+) 615 WP_162565571.1 SIS domain-containing protein -
  E5P2_RS02390 (G3W92_RS02390) - 485420..486157 (+) 738 WP_162565572.1 BON domain-containing protein -
  E5P2_RS02395 (G3W92_RS02395) - 486264..487181 (-) 918 WP_162565573.1 NAD(P)-dependent oxidoreductase -
  E5P2_RS02400 (G3W92_RS02400) pilU 487222..488358 (-) 1137 WP_162565574.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5P2_RS02405 (G3W92_RS02405) pilT 488393..489436 (-) 1044 WP_162565575.1 type IV pilus twitching motility protein PilT Machinery gene
  E5P2_RS02410 (G3W92_RS02410) - 489476..490162 (+) 687 WP_162565576.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5P2_RS02415 (G3W92_RS02415) - 490286..491557 (+) 1272 WP_162565577.1 Glu/Leu/Phe/Val dehydrogenase -
  E5P2_RS02420 (G3W92_RS02420) - 491689..492321 (+) 633 WP_232080555.1 DUF2242 domain-containing protein -
  E5P2_RS02425 (G3W92_RS02425) - 492379..492612 (-) 234 WP_162565579.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41909.02 Da        Isoelectric Point: 6.2437

>NTDB_id=1128200 E5P2_RS02400 WP_162565574.1 487222..488358(-) (pilU) [Variovorax sp. SRS16]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDDLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLIPTEDGHGRIAAVEILLNTPLISDMIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAITFEDAIRNADSANDLRLQIKLNSQRARTPDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1128200 E5P2_RS02400 WP_162565574.1 487222..488358(-) (pilU) [Variovorax sp. SRS16]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGATCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCCGACTTCCCGCCTGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCCCAGCAGGCGCTCGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCCGTCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACCGGGATCGGGCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCGACCATCGATGACCTCGGCATGCCGCAGGTGCTCAAGGATGTGGCGATGACCAAGCGCGGCCTGACGA
TCCTGGTGGGCGCGACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTGACGGTCGAGGATCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTCGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCGGTCGCCTTCGCCGAGACCGGGCATCTGTGCATGGCCACCCTGCATGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAATTTCTTTCCCGAGGAACGCCGGGCCCAGTTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATCTCGCAGCGCCTCATCCCGACCGAGGATGGGCACGGCCGCATCGCTGCGGTCGAAATCCTGCTCAACA
CGCCGCTGATCTCCGACATGATCTTCAAGGGCGAGGTGGGCGAAATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGG
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGGCAACGCGATCACCTTCGAGGATGCGATCCGCAACGCGGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCACGCCCGATCTGTCGGCAGGCACGGAAC
ATTTCGCAATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B6LF35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.824

94.444

0.556
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain Lp02

43.769

87.037

0.381
  pilT Legionella pneumophila strain ERS1305867

43.769

87.037

0.381
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment