Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5P3_RS03110 Genome accession   NZ_LR594662
Coordinates   651017..652060 (-) Length   347 a.a.
NCBI ID   WP_162578250.1    Uniprot ID   A0A6P2DV11
Organism   Variovorax sp. RA8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646017..657060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P3_RS03085 (G3W91_RS03085) - 646828..647193 (+) 366 WP_162589510.1 YraN family protein -
  E5P3_RS03090 (G3W91_RS03090) - 647286..647915 (+) 630 WP_162584632.1 SIS domain-containing protein -
  E5P3_RS03095 (G3W91_RS03095) - 647912..648655 (+) 744 WP_162584633.1 BON domain-containing protein -
  E5P3_RS03100 (G3W91_RS03100) - 648757..649677 (-) 921 WP_162584634.1 NAD(P)-dependent oxidoreductase -
  E5P3_RS03105 (G3W91_RS03105) pilU 649713..650849 (-) 1137 WP_162584635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5P3_RS03110 (G3W91_RS03110) pilT 651017..652060 (-) 1044 WP_162578250.1 type IV pilus twitching motility protein PilT Machinery gene
  E5P3_RS03115 (G3W91_RS03115) - 652099..652785 (+) 687 WP_162584636.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5P3_RS03120 (G3W91_RS03120) - 652792..653271 (-) 480 WP_162584637.1 Lrp/AsnC ligand binding domain-containing protein -
  E5P3_RS03125 (G3W91_RS03125) - 653366..656329 (+) 2964 WP_162584638.1 L-glutamate gamma-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38097.82 Da        Isoelectric Point: 6.8844

>NTDB_id=1128182 E5P3_RS03110 WP_162578250.1 651017..652060(-) (pilT) [Variovorax sp. RA8]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMINYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVVSQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREGKVAQMYSSIQTGSGQGMQTLDQC
LTDLVRRNVISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1128182 E5P3_RS03110 WP_162578250.1 651017..652060(-) (pilT) [Variovorax sp. RA8]
GTGGACATCACCCAACTGCTGGCCTTCAGCGTGAAGAACAAGGCCTCGGACCTGCATCTATCGTCCGGGCTGCCACCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACGTCGACGCGCTCGACCACAAGGCCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCCCGCGGCGCGGCGGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATTTTCGGCGACCTCGCGCTCAAGCCTCGTGGCCTGGTGCTGGTGACCGGCCCGACGGGTT
CGGGCAAGTCCACCACGCTGGCCGCGATGATCAACTACCTCAACGAGACCGAATACGGCCACATCCTGACGGTGGAGGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTCTCCTTCGCCAA
CGCCCTGCGCTCGGCGCTGCGAGAGGATCCCGACGCGATCCTGGTCGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CGATGACCGCGGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCGGCCGCCAAGACGATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAGAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGGTCTCGCAGAC
CCTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACCTCGGCCATCCGCAACCTGA
TCCGCGAGGGCAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCAGCGGCCAGGGCATGCAGACGCTGGACCAGTGC
CTGACGGACCTCGTGCGGCGCAATGTCATTTCCGCGGCAGAAGCCCGCGGCAAGGCAAAGATCCCCGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2DV11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment