Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5P3_RS03105 Genome accession   NZ_LR594662
Coordinates   649713..650849 (-) Length   378 a.a.
NCBI ID   WP_162584635.1    Uniprot ID   -
Organism   Variovorax sp. RA8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644713..655849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P3_RS03075 (G3W91_RS03075) - 645097..645825 (+) 729 WP_162589509.1 glutathione S-transferase -
  E5P3_RS03080 (G3W91_RS03080) rsmI 645869..646804 (-) 936 WP_162584631.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  E5P3_RS03085 (G3W91_RS03085) - 646828..647193 (+) 366 WP_162589510.1 YraN family protein -
  E5P3_RS03090 (G3W91_RS03090) - 647286..647915 (+) 630 WP_162584632.1 SIS domain-containing protein -
  E5P3_RS03095 (G3W91_RS03095) - 647912..648655 (+) 744 WP_162584633.1 BON domain-containing protein -
  E5P3_RS03100 (G3W91_RS03100) - 648757..649677 (-) 921 WP_162584634.1 NAD(P)-dependent oxidoreductase -
  E5P3_RS03105 (G3W91_RS03105) pilU 649713..650849 (-) 1137 WP_162584635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5P3_RS03110 (G3W91_RS03110) pilT 651017..652060 (-) 1044 WP_162578250.1 type IV pilus twitching motility protein PilT Machinery gene
  E5P3_RS03115 (G3W91_RS03115) - 652099..652785 (+) 687 WP_162584636.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5P3_RS03120 (G3W91_RS03120) - 652792..653271 (-) 480 WP_162584637.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42034.29 Da        Isoelectric Point: 6.5043

>NTDB_id=1128181 E5P3_RS03105 WP_162584635.1 649713..650849(-) (pilU) [Variovorax sp. RA8]
MERDQASQFINELLKLMVSRNGSDLFITADFPPAIKIDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVTMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRSQLLMDLSLNLRSLISQRLIPTEDGRGRIAAVEILLNTPLITDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFETNMISFEDALRNADSANDLRLQIKLNSQRARTTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1128181 E5P3_RS03105 WP_162584635.1 649713..650849(-) (pilU) [Variovorax sp. RA8]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGAACTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCCGCGATCAAGATCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCATTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTCAATGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
GGCCAAGCTCCCCACCATCGACGGGCTGGGGATGCCGCAGGTGCTGAAGGACGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAATTCCTTCGGC
CACATCGTGACGGTGGAGGACCCGGTAGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCAT
CGACACCGACAGCTGGGAGGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGAGAGACCATGGAGCATGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCTCCCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCTCGCTGATCTCGCAGCGCCTGATCCCGACCGAGGACGGCCGCGGCCGCATCGCCGCGGTGGAGATCCTGCTGAACA
CGCCGCTGATCACCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGACCAACATGATTTCCTTCGAGGACGCGCTGCGCAACGCCGA
CTCGGCCAATGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCACGACCGACCTGTCCGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.857

92.593

0.582
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.478

89.683

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.862

0.368
  pilT Vibrio cholerae strain A1552

43.849

83.862

0.368

Multiple sequence alignment