Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PMYSY11_RS02120 Genome accession   NZ_LR215729
Coordinates   486575..487609 (+) Length   344 a.a.
NCBI ID   WP_069902448.1    Uniprot ID   -
Organism   Pseudomonas marincola strain YSy11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481575..492609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMYSY11_RS02095 (PMYSY11_0442) - 481805..483787 (-) 1983 WP_090513818.1 dynamin-like GTPase family protein -
  PMYSY11_RS02100 (PMYSY11_0443) - 484006..484302 (-) 297 WP_150548184.1 DUF167 domain-containing protein -
  PMYSY11_RS02105 (PMYSY11_0444) - 484302..484892 (-) 591 WP_069902445.1 YggT family protein -
  PMYSY11_RS02110 (PMYSY11_0445) proC 484903..485721 (-) 819 WP_069902446.1 pyrroline-5-carboxylate reductase -
  PMYSY11_RS02115 (PMYSY11_0446) - 485837..486511 (-) 675 WP_090513814.1 YggS family pyridoxal phosphate-dependent enzyme -
  PMYSY11_RS02120 (PMYSY11_0447) pilT 486575..487609 (+) 1035 WP_069902448.1 type IV pilus twitching motility protein PilT Machinery gene
  PMYSY11_RS02125 (PMYSY11_0448) pilU 487787..488932 (+) 1146 WP_069902449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PMYSY11_RS02130 (PMYSY11_0449) - 489068..489466 (+) 399 WP_069902450.1 NINE protein -
  PMYSY11_RS02135 (PMYSY11_0450) egtB 489533..491686 (-) 2154 WP_150548185.1 ergothioneine biosynthesis protein EgtB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37960.73 Da        Isoelectric Point: 6.7750

>NTDB_id=1125914 PMYSY11_RS02120 WP_069902448.1 486575..487609(+) (pilT) [Pseudomonas marincola strain YSy11]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSRVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNTTKYQHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KTLVSKGLIGRDMAKEKAKNPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1125914 PMYSY11_RS02120 WP_069902448.1 486575..487609(+) (pilT) [Pseudomonas marincola strain YSy11]
ATGGATATCACTGAGCTGCTCGCCTTCAGTGCCAAGCAAGGTGCGTCGGACCTGCACCTCTCTGCTGGTTTGCCGCCGAT
GATTCGGGTTGATGGCGATGTTCGCCGCATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAAGAGTTTCTGGAAACGGACTTCTCCTTTGAAGTGCCAGGCGTTGCCCGT
TTCCGGGTTAACGCATTCAACCAGAATCGCGGTTCGGGCGCAGTATTCCGGACGATTCCGTCGCGGGTTCTGACCATGGA
AGACCTCGGCATGGGTGAGGTGTTCCGCAAGATCACCGACGTGCCGCGCGGTCTGGTACTGGTTACAGGGCCAACCGGTT
CGGGTAAATCGACCACGCTGGCGGCGATGCTCGATTACCTCAACACCACCAAGTACCAACATATTCTGACCATCGAAGAC
CCGATCGAATTCGTTCACGAGTCGAAGAAGTGTCTGGTCAACCAACGTGAAGTACACCGTGACACGCTTGGCTTCTCCGA
AGCATTGCGCTCGGCGCTGCGTGAAGACCCGGATATCATTCTGGTCGGTGAATTGCGCGATCTGGAAACCATTCGTTTGG
CCCTGACCGCTGCTGAAACCGGCCACCTGGTGTTCGGTACGCTGCACACCACCTCTGCTGCCAAGACCATCGACCGTGTG
GTTGACGTGTTCCCGGCGGAAGAGAAGTCGATGGTTCGCTCGATGCTGTCCGAATCATTGCAGGCGGTGATTTCGCAAAC
CCTGCTGAAGAAAATCGGCGGTGGGCGTGTTGCCGCCCACGAAATCATGATCGGCACGCCGGCGATTCGTAACCTGATCC
GTGAAGACAAGGTTGCGCAGATGTATTCCGCCATCCAGACCGGTGGCTCGCTGGGCATGCAAACCCTGGATTCGTGCCTG
AAAACCTTGGTCAGCAAAGGCCTGATCGGCCGAGACATGGCCAAAGAGAAGGCTAAAAACCCAGAGAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.64

100

0.956
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

73.837

100

0.738
  pilT Legionella pneumophila strain ERS1305867

73.837

100

0.738
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.64

0.672
  pilT Vibrio cholerae strain A1552

70.213

95.64

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

42.462

94.477

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.93

0.392
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment