Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PMYSY11_RS02125 Genome accession   NZ_LR215729
Coordinates   487787..488932 (+) Length   381 a.a.
NCBI ID   WP_069902449.1    Uniprot ID   A0A1I7D768
Organism   Pseudomonas marincola strain YSy11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482787..493932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMYSY11_RS02100 (PMYSY11_0443) - 484006..484302 (-) 297 WP_150548184.1 DUF167 domain-containing protein -
  PMYSY11_RS02105 (PMYSY11_0444) - 484302..484892 (-) 591 WP_069902445.1 YggT family protein -
  PMYSY11_RS02110 (PMYSY11_0445) proC 484903..485721 (-) 819 WP_069902446.1 pyrroline-5-carboxylate reductase -
  PMYSY11_RS02115 (PMYSY11_0446) - 485837..486511 (-) 675 WP_090513814.1 YggS family pyridoxal phosphate-dependent enzyme -
  PMYSY11_RS02120 (PMYSY11_0447) pilT 486575..487609 (+) 1035 WP_069902448.1 type IV pilus twitching motility protein PilT Machinery gene
  PMYSY11_RS02125 (PMYSY11_0448) pilU 487787..488932 (+) 1146 WP_069902449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PMYSY11_RS02130 (PMYSY11_0449) - 489068..489466 (+) 399 WP_069902450.1 NINE protein -
  PMYSY11_RS02135 (PMYSY11_0450) egtB 489533..491686 (-) 2154 WP_150548185.1 ergothioneine biosynthesis protein EgtB -
  PMYSY11_RS02140 (PMYSY11_0451) - 491988..493259 (-) 1272 WP_150548186.1 dihydroorotase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42408.60 Da        Isoelectric Point: 6.8331

>NTDB_id=1125915 PMYSY11_RS02125 WP_069902449.1 487787..488932(+) (pilU) [Pseudomonas marincola strain YSy11]
MDFEKLLRLMVEKGGSDLFITAGIPPSMKVNGKILPITKTPMSPEQTRETVHAVMNDQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEELKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQSQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHQLKELMKRSSEQGMQTFDQA
LYKLYSDGEITYENALAYADSANDLRLMIKLASEGDGDHLSSLTEGLSLEVSTDDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1125915 PMYSY11_RS02125 WP_069902449.1 487787..488932(+) (pilU) [Pseudomonas marincola strain YSy11]
ATGGACTTTGAAAAACTATTACGTCTGATGGTCGAAAAGGGCGGCTCCGATCTTTTCATTACAGCCGGTATTCCGCCATC
GATGAAGGTCAATGGCAAAATCTTGCCGATCACCAAAACGCCGATGTCACCCGAGCAAACCCGCGAAACAGTGCACGCGG
TCATGAATGATCAGCAGCGCCGTGACTTTGCCGAGAACCATGAGTGCAACTTTGCAATCAGCGCTCGCGGAATCGGGCGG
TTCCGGGTGAGTGCCTTCTACCAGCGCAACCTGGCTGGCATGGTGCTGCGCCGTATCGAAACCAACATCCCCACGCTCGA
AGAGTTGAAACTGCCGGAAGTGCTGAAAAAACTGGCCCTGACCAAGCGTGGTCTGGTGTTGTTTGTGGGCGCTACCGGTA
CCGGTAAATCGACCTCGCTGGCGGCGATGATTGGCTATCGCAACAAGAACAGCAGCGGCCACATCATTTCCATCGAAGAC
CCGATCGAATTCATTCACCAGCACCAGAATTGCATCGTCACTCAGCGCGAAGTCGGCATCGATACCGAATCGTTCGAAGT
GGCGCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGATCTTGATTGGTGAGGTGCGCTCGCGCGAGACCATGGATCACG
CCGTCGCATTTGCCGAAACCGGCCACCTGTGTCTGGCCACATTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCAACTTCTTCCCGGCAGACCGGCAAAGCCAGGTCTGGATGGACCTGTCGCTTAACCTCAAGGCAATTGTCGCTCAGCA
ACTGATCCCGACACCCGACGGCAAAGGCCGCCGCGCGGTGATTGAAGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATCAGCTCAAGGAGTTGATGAAGCGTTCTTCTGAGCAGGGCATGCAGACGTTCGATCAGGCT
CTGTACAAGCTCTACAGCGACGGTGAAATCACTTACGAAAACGCCTTGGCCTATGCCGACTCGGCGAACGACTTGCGCCT
GATGATCAAGCTGGCTTCCGAAGGTGACGGCGATCATTTGTCGAGTCTCACCGAAGGCTTGTCGCTTGAAGTCAGTACCG
ATGATCCGGGCCGTCGCTTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I7D768

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

89.764

100

0.898
  pilU Acinetobacter baylyi ADP1

64.146

93.701

0.601
  pilU Vibrio cholerae strain A1552

60.286

91.864

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

93.701

0.402
  pilT Pseudomonas aeruginosa PAK

43.284

87.927

0.381
  pilT Acinetobacter baylyi ADP1

41.935

89.501

0.375
  pilT Pseudomonas stutzeri DSM 10701

42.388

87.927

0.373
  pilT Legionella pneumophila strain ERS1305867

42.388

87.927

0.373
  pilT Legionella pneumophila strain Lp02

42.388

87.927

0.373
  pilT Neisseria meningitidis 8013

41.159

90.551

0.373
  pilT Neisseria gonorrhoeae MS11

41.159

90.551

0.373
  pilT Acinetobacter nosocomialis M2

41.791

87.927

0.367
  pilT Acinetobacter baumannii D1279779

41.493

87.927

0.365
  pilT Acinetobacter baumannii strain A118

41.493

87.927

0.365

Multiple sequence alignment