Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   S7S_RS18125 Genome accession   NZ_CP004387
Coordinates   4029799..4030833 (-) Length   344 a.a.
NCBI ID   WP_008734599.1    Uniprot ID   -
Organism   Isoalcanivorax pacificus W11-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4024799..4035833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S7S_RS18105 (S7S_18030) - 4025668..4026648 (+) 981 WP_008734606.1 glutathione S-transferase family protein -
  S7S_RS18110 (S7S_18035) - 4026674..4027528 (+) 855 WP_008734604.1 pirin family protein -
  S7S_RS18115 (S7S_18040) - 4027563..4028588 (-) 1026 WP_008734602.1 NAD(P)H-dependent flavin oxidoreductase -
  S7S_RS18120 (S7S_18045) pilU 4028645..4029766 (-) 1122 WP_008734601.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  S7S_RS18125 (S7S_18050) pilT 4029799..4030833 (-) 1035 WP_008734599.1 type IV pilus twitching motility protein PilT Machinery gene
  S7S_RS18130 (S7S_18055) - 4031004..4031708 (+) 705 WP_008734597.1 YggS family pyridoxal phosphate-dependent enzyme -
  S7S_RS18135 (S7S_18060) proC 4031802..4032665 (+) 864 WP_052269278.1 pyrroline-5-carboxylate reductase -
  S7S_RS18140 (S7S_18065) - 4032738..4033280 (+) 543 WP_008734594.1 YggT family protein -
  S7S_RS18145 (S7S_18070) - 4033280..4033567 (+) 288 WP_008734591.1 DUF167 family protein -
  S7S_RS18150 (S7S_18075) uvrD 4033641..4035818 (-) 2178 WP_008734590.1 DNA helicase II -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37961.66 Da        Isoelectric Point: 6.4206

>NTDB_id=112335 S7S_RS18125 WP_008734599.1 4029799..4030833(-) (pilT) [Isoalcanivorax pacificus W11-5]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKEVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGKVFQKIAETPRGIVLVTGPTGSGKSTTLAAMIDFINNTRYEHILTIED
PIEFVHESKKCLMNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAGLGMQTLDQCL
QGLVQKGLISREAAREKAKTPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=112335 S7S_RS18125 WP_008734599.1 4029799..4030833(-) (pilT) [Isoalcanivorax pacificus W11-5]
ATGGATATCACCGAACTGCTTGCCTTCAGCGCCAAGAACGGCGCGTCTGACCTTCACCTGTCGGCGGGCCTGCCGCCAAT
GATCCGGGTGGACGGCGACGTCCGCCGCATCAACCTGCCGCCGATGGAACACAAGGAAGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTATGAAGAGTTCCTGGAAACCGACTTCTCGTTCGAGGTGCCCGGCGTGGCCCGC
TTCCGGGTCAACGCCTTTAACCAGAACCGTGGCGCCGGCGCCGTATTCCGGACCATTCCGTCGAAGGTACTGTCGATGGA
AGACCTAGGCATGGGCAAGGTGTTCCAGAAGATCGCCGAAACCCCGCGCGGCATCGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAATCGACCACGCTGGCGGCCATGATCGACTTCATCAACAACACCCGCTACGAGCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAAAAGTGCCTGATGAACCAGCGGGAAGTGCACCGCGACACGCTGGGCTTCGCCGA
AGCACTGCGCTCGGCGCTACGGGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGCGACCTGGAAACCATCCGGCTGG
CGCTGACCGCTGCGGAAACCGGGCACCTTGTGTTCGGCACCTTGCACACCACCTCCGCGGCGAAAACCATTGATCGGGTG
GTGGACGTGTTCCCGGCGGAAGAAAAATCCATGGTGCGCTCGATGCTGTCGGAATCCCTGCAGGCGGTCATCTCGCAGAC
CCTGCTGAAGAAAAACGGCGGTGGCCGGGTGGCCGCGCACGAAATCATGATCGGCACCCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCGCCGGCCTGGGTATGCAGACACTGGACCAGTGTCTG
CAGGGGCTGGTGCAGAAAGGCCTGATCTCCCGCGAAGCGGCCCGCGAAAAAGCAAAAACGCCGGATTCCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

90.116

100

0.901
  pilT Pseudomonas stutzeri DSM 10701

90.116

100

0.901
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baumannii D1279779

85.465

100

0.855
  pilT Acinetobacter baumannii strain A118

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

76.163

100

0.762
  pilT Legionella pneumophila strain ERS1305867

76.163

100

0.762
  pilT Neisseria gonorrhoeae MS11

71.014

100

0.712
  pilT Neisseria meningitidis 8013

71.014

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment