Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   S7S_RS18120 Genome accession   NZ_CP004387
Coordinates   4028645..4029766 (-) Length   373 a.a.
NCBI ID   WP_008734601.1    Uniprot ID   A0A0B4XS73
Organism   Isoalcanivorax pacificus W11-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4023645..4034766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S7S_RS18090 (S7S_18015) - 4023756..4024286 (-) 531 WP_169745583.1 DUF4136 domain-containing protein -
  S7S_RS18095 (S7S_18020) - 4024402..4024629 (-) 228 WP_008734610.1 DUF1653 domain-containing protein -
  S7S_RS18100 (S7S_18025) - 4024622..4025563 (-) 942 WP_008734608.1 LysR family transcriptional regulator -
  S7S_RS18105 (S7S_18030) - 4025668..4026648 (+) 981 WP_008734606.1 glutathione S-transferase family protein -
  S7S_RS18110 (S7S_18035) - 4026674..4027528 (+) 855 WP_008734604.1 pirin family protein -
  S7S_RS18115 (S7S_18040) - 4027563..4028588 (-) 1026 WP_008734602.1 NAD(P)H-dependent flavin oxidoreductase -
  S7S_RS18120 (S7S_18045) pilU 4028645..4029766 (-) 1122 WP_008734601.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  S7S_RS18125 (S7S_18050) pilT 4029799..4030833 (-) 1035 WP_008734599.1 type IV pilus twitching motility protein PilT Machinery gene
  S7S_RS18130 (S7S_18055) - 4031004..4031708 (+) 705 WP_008734597.1 YggS family pyridoxal phosphate-dependent enzyme -
  S7S_RS18135 (S7S_18060) proC 4031802..4032665 (+) 864 WP_052269278.1 pyrroline-5-carboxylate reductase -
  S7S_RS18140 (S7S_18065) - 4032738..4033280 (+) 543 WP_008734594.1 YggT family protein -
  S7S_RS18145 (S7S_18070) - 4033280..4033567 (+) 288 WP_008734591.1 DUF167 family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41523.08 Da        Isoelectric Point: 7.1072

>NTDB_id=112334 S7S_RS18120 WP_008734601.1 4028645..4029766(-) (pilU) [Isoalcanivorax pacificus W11-5]
MEFEELLKIMVQKGGSDLFITAGVPPSMKIHGKVVPVTKTALTPDMTRDIVFGVMTEKQRKEFIESKECNFAISARGIGR
FRVSAFYQRNLVGMVIRRIEVKIPSVDELRLPPVIKDLAMTKRGLVIFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGLDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQLYMDLSLNLRGIVAQQLIATPDGKGRRAVIEVLLNTPLMADHIRKGEVHLLKELMKKSRDLGMQTFDQA
LYDLYTQGEITYEDAMMHADAPNDLRLMIKLGAETSSSSLDSATRGLSIEDTE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=112334 S7S_RS18120 WP_008734601.1 4028645..4029766(-) (pilU) [Isoalcanivorax pacificus W11-5]
ATGGAATTTGAAGAACTCCTGAAAATCATGGTGCAGAAGGGCGGCTCGGATCTGTTTATCACCGCCGGCGTGCCACCTTC
GATGAAAATCCACGGCAAGGTGGTGCCCGTTACCAAAACGGCACTGACACCGGACATGACGCGCGACATCGTATTCGGCG
TGATGACCGAGAAACAGCGCAAGGAGTTCATCGAAAGCAAGGAATGCAACTTCGCCATCTCGGCGCGCGGTATCGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTCGGCATGGTGATCCGCCGCATCGAAGTGAAAATCCCGTCCGTGGA
CGAGCTGCGCCTGCCGCCGGTGATCAAGGACCTGGCGATGACCAAGCGCGGCCTGGTGATATTCGTGGGTGCCACCGGCA
CCGGTAAATCGACCTCGCTGGCGTCCATGATTGGCCACCGCAACCGCAATTCCAAAGGCCATATCATCTCCATCGAAGAC
CCGATCGAATTCATTCACCAGCACCAGGGTTGCATCGTCACCCAGCGTGAAGTGGGTCTGGACACGGATTCCTTCGAGAT
CGCCCTGAAGAACACTCTGCGCCAGGCGCCGGACGTCATCCTGATCGGCGAGATCCGCTCCCGCGAGGTAATGGACTACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACACTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCCACTTCTTCCCCGCCGACCGGCACGGCCAGCTCTACATGGACCTGTCGCTGAACCTGCGCGGCATCGTGGCACAGCA
ACTGATTGCCACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAAGTACTGTTGAACACGCCGCTGATGGCGGACCACA
TCCGCAAGGGCGAAGTGCATCTCTTGAAAGAGCTGATGAAAAAGTCCCGCGACCTCGGCATGCAGACCTTCGACCAGGCG
CTCTATGATCTCTACACCCAGGGCGAGATCACCTACGAAGACGCCATGATGCACGCCGACGCGCCGAACGACCTGCGCCT
GATGATCAAGCTGGGGGCGGAGACCAGTTCCAGTTCGCTGGATTCGGCGACGCGCGGGTTGTCGATCGAAGATACCGAGT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B4XS73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.108

99.196

0.775
  pilU Acinetobacter baylyi ADP1

67.385

99.464

0.67
  pilU Vibrio cholerae strain A1552

55.22

97.587

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.835

97.587

0.389

Multiple sequence alignment