Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL320_RS12045 Genome accession   NZ_LR134493
Coordinates   2491896..2492888 (+) Length   330 a.a.
NCBI ID   WP_126531454.1    Uniprot ID   A0A3S4WVY9
Organism   Serratia rubidaea strain NCTC10036     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2486896..2497888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL320_RS12015 (NCTC10036_02376) hemW 2487710..2488861 (-) 1152 WP_054305190.1 radical SAM family heme chaperone HemW -
  EL320_RS12020 (NCTC10036_02377) - 2488851..2489447 (-) 597 WP_126531452.1 XTP/dITP diphosphatase -
  EL320_RS12025 (NCTC10036_02378) yggU 2489465..2489755 (-) 291 WP_015673798.1 DUF167 family protein YggU -
  EL320_RS12030 (NCTC10036_02379) - 2489752..2490306 (-) 555 WP_015673797.1 YggT family protein -
  EL320_RS12035 (NCTC10036_02380) proC 2490326..2491147 (-) 822 WP_054305191.1 pyrroline-5-carboxylate reductase -
  EL320_RS12040 (NCTC10036_02381) - 2491168..2491875 (-) 708 WP_126531453.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL320_RS12045 (NCTC10036_02382) pilT 2491896..2492888 (+) 993 WP_126531454.1 type IV pilus twitching motility protein PilT Machinery gene
  EL320_RS12050 (NCTC10036_02383) - 2492965..2493666 (+) 702 WP_126531455.1 response regulator transcription factor -
  EL320_RS12055 (NCTC10036_02384) - 2493659..2497195 (+) 3537 WP_164722780.1 ATP-binding protein -
  EL320_RS12060 (NCTC10036_02385) - 2497297..2497740 (+) 444 WP_232037741.1 DUF1090 domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35699.07 Da        Isoelectric Point: 7.0088

>NTDB_id=1123226 EL320_RS12045 WP_126531454.1 2491896..2492888(+) (pilT) [Serratia rubidaea strain NCTC10036]
MDIDEFVALSVKHNASDLHLCTGQPLMMRIDGELQPLAGGAVLPAEAVQRLCAALLDEPQQRQLRRAGQLDCALTTAGGT
RLRANFFYQRAGVSLALRRIAECCPTLEQLQAPAIIPALTEREEGLILVTGATGSGKSTTLAAMIDHINRHQRRHILTLE
DPIEFIHHSALSLIQQRELGRDTRSFDAALSAALREDPDVILVGELRDSLSIRLALTAAETGHLVLATLHTRNAPQAVER
VIDVFPAEEKAYVRAQLASGLQAVIAQKLLRRAEGGRTAVFEILTATPAVSSMIREGKTHQLASVLQSGAQAGMQTFAQG
MQQRIAQGIL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=1123226 EL320_RS12045 WP_126531454.1 2491896..2492888(+) (pilT) [Serratia rubidaea strain NCTC10036]
ATGGATATCGATGAATTTGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACCGGCCAACCGTTGAT
GATGCGCATCGATGGTGAACTTCAGCCGCTGGCGGGCGGCGCCGTCCTGCCCGCCGAGGCGGTGCAGCGGCTGTGCGCGG
CGCTGCTTGACGAGCCTCAGCAGCGGCAATTACGGCGCGCCGGGCAGCTGGATTGCGCATTGACAACCGCGGGCGGCACG
CGGCTGCGCGCCAACTTTTTTTATCAGCGCGCCGGCGTGTCGCTGGCGCTGCGTCGTATTGCGGAATGCTGTCCGACGCT
GGAGCAGCTGCAGGCGCCGGCGATTATCCCGGCGCTCACCGAACGGGAAGAGGGGCTGATTCTGGTCACGGGCGCGACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATCGACCATATCAACCGTCATCAGCGGCGGCATATTCTGACGCTGGAA
GACCCGATTGAGTTTATCCACCACAGCGCGCTGTCGCTGATTCAGCAGCGCGAACTGGGGCGTGACACCCGCAGCTTCGA
CGCGGCGCTGAGCGCCGCGCTGCGCGAAGATCCCGACGTGATCCTGGTCGGCGAGCTGCGCGACAGCCTCAGCATCCGGC
TGGCGCTGACCGCCGCGGAAACCGGCCACCTGGTGCTGGCGACCCTGCACACCCGCAACGCGCCGCAGGCGGTTGAGCGG
GTGATCGATGTCTTCCCGGCGGAGGAGAAAGCCTACGTGCGTGCCCAACTGGCCAGCGGTCTGCAGGCGGTCATCGCGCA
GAAATTACTGCGCAGGGCCGAAGGCGGCAGAACGGCGGTGTTTGAGATCCTGACGGCGACGCCGGCGGTAAGCAGCATGA
TCCGTGAAGGAAAAACGCATCAGCTGGCCAGCGTGCTGCAAAGTGGCGCGCAGGCGGGCATGCAAACCTTTGCTCAGGGA
ATGCAGCAACGTATTGCGCAGGGAATTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4WVY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.727

100

0.527
  pilT Vibrio cholerae strain A1552

52.727

100

0.527
  pilT Acinetobacter baylyi ADP1

49.697

100

0.497
  pilT Acinetobacter baumannii strain A118

49.249

100

0.497
  pilT Acinetobacter baumannii D1279779

49.249

100

0.497
  pilT Acinetobacter nosocomialis M2

48.949

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.503

100

0.491
  pilT Neisseria meningitidis 8013

48.204

100

0.488
  pilT Pseudomonas stutzeri DSM 10701

47.447

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.273

100

0.473
  pilT Legionella pneumophila strain Lp02

47.273

100

0.473
  pilT Pseudomonas aeruginosa PAK

46.547

100

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.484

96.364

0.458
  pilU Vibrio cholerae strain A1552

41.358

98.182

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.818

99.697

0.397
  pilU Acinetobacter baylyi ADP1

39.319

97.879

0.385

Multiple sequence alignment