Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL249_RS11525 Genome accession   NZ_LR134378
Coordinates   2811415..2812458 (-) Length   347 a.a.
NCBI ID   WP_040529606.1    Uniprot ID   -
Organism   Lautropia mirabilis strain NCTC12852     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2806415..2817458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL249_RS11505 (NCTC12852_02288) - 2806600..2807964 (-) 1365 WP_232002019.1 glycerophosphodiester phosphodiesterase -
  EL249_RS11510 (NCTC12852_02289) - 2808088..2808930 (-) 843 WP_040530491.1 carbonic anhydrase -
  EL249_RS11515 (NCTC12852_02290) - 2809227..2810042 (-) 816 WP_005672178.1 carbonic anhydrase -
  EL249_RS11520 (NCTC12852_02291) pilU 2810161..2811294 (-) 1134 WP_040530489.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL249_RS11525 (NCTC12852_02292) pilT 2811415..2812458 (-) 1044 WP_040529606.1 type IV pilus twitching motility protein PilT Machinery gene
  EL249_RS11530 (NCTC12852_02293) - 2813059..2813769 (+) 711 WP_005672170.1 autoinducer binding domain-containing protein -
  EL249_RS11535 (NCTC12852_02294) - 2814046..2814759 (+) 714 WP_005672168.1 autoinducer binding domain-containing protein -
  EL249_RS11540 (NCTC12852_02295) - 2815140..2816153 (+) 1014 WP_005672167.1 metal ABC transporter substrate-binding protein -
  EL249_RS11545 (NCTC12852_02296) - 2816150..2817103 (+) 954 WP_005672165.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38139.92 Da        Isoelectric Point: 6.7216

>NTDB_id=1122473 EL249_RS11525 WP_040529606.1 2811415..2812458(-) (pilT) [Lautropia mirabilis strain NCTC12852]
MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMDHEEVHNLIYDIMNDSQRKEYEERLECDFSFSIPGLAR
FRVNAFVQERGAGAVMRTIPSKILTLEQLNCPKSFTDIAMQPRGIVLVTGPTGSGKSTTLAAMINHINENVYGHILTVED
PIEFVHESKRCLINQREVGPHTQSFNNALKSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRAMLSESLKAVISQTLLKTKDGNGRVAAHEIMIGTPAIRNLIREAKVSQMNAAIQTGGSQGMQTLDQC
LTDLVRKNVISMAEARAAAAVKENFPL

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1122473 EL249_RS11525 WP_040529606.1 2811415..2812458(-) (pilT) [Lautropia mirabilis strain NCTC12852]
ATGGACATCACAGAGCTGTTGGCGTTTTCGGTCAAGAACAAGGCGTCGGACCTGCACCTGTCGGCGGGGCTGCCGCCGAT
GATCCGGGTGCATGGCGACGTGCGGCGGATCAACCTGCCGGCGATGGATCATGAAGAGGTGCACAACCTCATCTACGACA
TCATGAACGACTCGCAGCGCAAGGAATACGAAGAGCGCCTGGAGTGCGACTTCTCGTTCTCGATTCCCGGCCTGGCACGT
TTCCGTGTGAACGCCTTCGTGCAGGAGCGGGGCGCCGGCGCGGTGATGCGTACGATTCCGTCCAAGATCCTCACGCTGGA
GCAGCTCAACTGCCCCAAATCCTTCACCGACATCGCCATGCAGCCGCGCGGCATCGTGCTGGTGACCGGTCCGACCGGCT
CCGGTAAGTCGACCACGCTGGCGGCGATGATCAACCACATCAACGAGAACGTCTACGGTCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACGAGTCCAAGCGCTGCCTGATCAACCAGCGCGAAGTGGGGCCGCACACCCAGTCGTTCAACAA
CGCGCTCAAGAGCGCGCTGCGTGAAGACCCGGACATCATCCTGGTGGGTGAGCTTCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCCGCAGCCAAGACCATCGACCGTATC
GTGGACGTGTTCCCGGCAGCCGAGAAGGACATGGTGCGCGCCATGCTGTCCGAATCGCTGAAGGCCGTCATCTCGCAGAC
GCTGCTGAAGACCAAGGACGGCAACGGCCGTGTGGCGGCGCACGAGATCATGATCGGCACGCCCGCCATCCGGAACCTGA
TCCGCGAGGCGAAGGTGTCGCAGATGAACGCTGCCATCCAGACCGGCGGTTCGCAGGGCATGCAGACGCTGGATCAGTGT
CTGACCGACCTGGTCCGCAAGAACGTCATCTCGATGGCCGAGGCACGCGCCGCGGCTGCCGTGAAAGAGAACTTCCCGCT
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

71.304

99.424

0.709
  pilT Legionella pneumophila strain ERS1305867

71.304

99.424

0.709
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.118

0.654
  pilT Vibrio cholerae strain A1552

67.359

97.118

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.964

95.389

0.496
  pilU Acinetobacter baylyi ADP1

43.391

100

0.435
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Vibrio cholerae strain A1552

42.478

97.695

0.415

Multiple sequence alignment