Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL249_RS11520 Genome accession   NZ_LR134378
Coordinates   2810161..2811294 (-) Length   377 a.a.
NCBI ID   WP_040530489.1    Uniprot ID   -
Organism   Lautropia mirabilis strain NCTC12852     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2805161..2816294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL249_RS11505 (NCTC12852_02288) - 2806600..2807964 (-) 1365 WP_232002019.1 glycerophosphodiester phosphodiesterase -
  EL249_RS11510 (NCTC12852_02289) - 2808088..2808930 (-) 843 WP_040530491.1 carbonic anhydrase -
  EL249_RS11515 (NCTC12852_02290) - 2809227..2810042 (-) 816 WP_005672178.1 carbonic anhydrase -
  EL249_RS11520 (NCTC12852_02291) pilU 2810161..2811294 (-) 1134 WP_040530489.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL249_RS11525 (NCTC12852_02292) pilT 2811415..2812458 (-) 1044 WP_040529606.1 type IV pilus twitching motility protein PilT Machinery gene
  EL249_RS11530 (NCTC12852_02293) - 2813059..2813769 (+) 711 WP_005672170.1 autoinducer binding domain-containing protein -
  EL249_RS11535 (NCTC12852_02294) - 2814046..2814759 (+) 714 WP_005672168.1 autoinducer binding domain-containing protein -
  EL249_RS11540 (NCTC12852_02295) - 2815140..2816153 (+) 1014 WP_005672167.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41882.32 Da        Isoelectric Point: 6.9783

>NTDB_id=1122472 EL249_RS11520 WP_040530489.1 2810161..2811294(-) (pilU) [Lautropia mirabilis strain NCTC12852]
MEREQASAFLYDLLRLMIAKDGSDLFLTVDFPPAFKISGKMVPVSDKPLTTQHTIELARALMNDRQAAEFEANKELNFAI
SPGGIGRFRVNAFTQMGRTGLVLRVIKSEIPDLATLNLPPVMSELSMTQRGLILMVGATGSGKSTTLAAMIGHRNEHSHG
HIITIEDPVEFVHVHKNCIVTHREVGVDTLDWHVALKNTLRQAPDVIMLGEIRDRETMEHAVAFAETGHLCMGTLHANSA
NQALDRIINFFPDERRQQLLMDLSLNLKAVISQRLVPAEGRKGRVAAVEIMINSPLISDLIFKGSVSEIKEIMKKSRELG
MQTFDQHLFDLFEAGQISYENALRNADSVNDLRLNIKLNSKRRSEDPTAASSKLGIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1122472 EL249_RS11520 WP_040530489.1 2810161..2811294(-) (pilU) [Lautropia mirabilis strain NCTC12852]
ATGGAACGTGAGCAGGCGTCGGCCTTCCTGTACGACCTGCTGCGGCTGATGATCGCCAAGGACGGTTCGGACCTGTTCCT
GACCGTGGACTTCCCGCCGGCCTTCAAGATCAGCGGCAAGATGGTGCCGGTCTCGGACAAGCCGCTGACCACCCAGCACA
CCATCGAGCTGGCGCGGGCGCTGATGAACGACCGCCAGGCGGCCGAGTTCGAGGCCAACAAGGAACTGAACTTCGCCATC
AGCCCGGGCGGCATCGGCCGCTTCCGGGTCAATGCCTTCACGCAGATGGGGCGCACGGGCCTGGTGCTGCGGGTGATCAA
GAGCGAGATTCCCGATCTGGCCACCCTGAACCTGCCGCCGGTGATGAGCGAGCTGTCGATGACGCAGCGCGGCCTGATCC
TGATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACGCTGGCCGCGATGATCGGCCACCGCAACGAGCACAGCCACGGC
CACATCATCACCATCGAGGACCCGGTGGAATTCGTGCACGTGCACAAGAACTGCATCGTCACACACCGCGAGGTGGGTGT
CGACACGCTGGACTGGCACGTCGCACTGAAGAACACGCTGCGTCAGGCGCCCGACGTGATCATGCTGGGCGAGATCCGTG
ACCGCGAGACGATGGAACATGCGGTGGCCTTTGCCGAGACCGGCCACCTGTGCATGGGCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGCATCATCAACTTCTTCCCCGACGAGCGCCGCCAGCAGCTGCTGATGGATCTGTCGCTGAACCT
GAAGGCGGTGATCTCGCAGCGCCTGGTGCCGGCCGAAGGGCGCAAGGGGCGCGTGGCTGCGGTGGAGATCATGATCAACT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCAGCGTCAGCGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGCACCTCTTCGACCTGTTCGAGGCCGGGCAGATCAGCTACGAGAACGCACTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGAACATCAAGCTCAACAGCAAGCGCCGGAGCGAGGATCCCACCGCGGCCAGCAGCAAGC
TGGGCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.773

96.021

0.584
  pilU Acinetobacter baylyi ADP1

55.84

93.103

0.52
  pilU Vibrio cholerae strain A1552

52.113

94.164

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.39

0.403
  pilT Pseudomonas aeruginosa PAK

42.73

89.39

0.382
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.39

0.371
  pilT Legionella pneumophila strain Lp02

41.742

88.329

0.369
  pilT Legionella pneumophila strain ERS1305867

41.742

88.329

0.369
  pilT Acinetobacter baylyi ADP1

41.441

88.329

0.366

Multiple sequence alignment