Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL255_RS02805 Genome accession   NZ_LR134376
Coordinates   592615..593649 (-) Length   344 a.a.
NCBI ID   WP_042652087.1    Uniprot ID   -
Organism   Aeromonas encheleia strain NCTC12917     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 587615..598649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL255_RS02785 (NCTC12917_00565) - 588430..589140 (-) 711 WP_042652083.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  EL255_RS02790 (NCTC12917_00566) srmB 589269..590489 (+) 1221 WP_042652084.1 ATP-dependent RNA helicase SrmB -
  EL255_RS02795 (NCTC12917_00567) yaaA 590675..591448 (-) 774 WP_042652085.1 peroxide stress protein YaaA -
  EL255_RS02800 (NCTC12917_00568) pilU 591480..592589 (-) 1110 WP_042652086.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL255_RS02805 (NCTC12917_00569) pilT 592615..593649 (-) 1035 WP_042652087.1 type IVa pilus ATPase TapT Machinery gene
  EL255_RS02810 (NCTC12917_00570) - 593689..594390 (+) 702 WP_042652088.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL255_RS02815 (NCTC12917_00571) proC 594474..595298 (+) 825 WP_042652089.1 pyrroline-5-carboxylate reductase -
  EL255_RS02820 (NCTC12917_00572) - 595328..595879 (+) 552 WP_042652090.1 YggT family protein -
  EL255_RS02825 (NCTC12917_00573) yggU 595879..596172 (+) 294 WP_042652091.1 DUF167 family protein YggU -
  EL255_RS02830 (NCTC12917_00574) - 596192..596611 (+) 420 WP_042652092.1 DUF4426 domain-containing protein -
  EL255_RS02835 (NCTC12917_00575) - 596695..597525 (-) 831 WP_042652093.1 CPBP family intramembrane glutamic endopeptidase -
  EL255_RS02840 (NCTC12917_00576) - 597721..598017 (-) 297 WP_005332293.1 hypothetical protein -
  EL255_RS02845 (NCTC12917_00577) - 598070..598444 (-) 375 WP_042652094.1 YacL family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38245.00 Da        Isoelectric Point: 6.7439

>NTDB_id=1122431 EL255_RS02805 WP_042652087.1 592615..593649(-) (pilT) [Aeromonas encheleia strain NCTC12917]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLGAPEMFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTRSFANALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGIVAALDAKAKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1122431 EL255_RS02805 WP_042652087.1 592615..593649(-) (pilT) [Aeromonas encheleia strain NCTC12917]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAAGAGAACTTCGAGGTGGATTTCTCCTTCGAGGTGCCCAACCTGGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTCCGCACCATCCCCAGTACAGTGCTGAGCCTGGA
GGATCTGGGGGCCCCGGAGATGTTTCGCAAGATCGCCGAATTCCCCCGCGGCCTGGTGCTGGTCACAGGCCCGACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAAAACAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCAGGAGTTTCGCCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGATCCGGACATCATACTGGTGGGGGAGATGCGCGATCTGGAGACCATACGTCTCG
CCATGACCGCCGCCGAGACCGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATAGACAGGATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTACGCTCCATGCTGTCCGAATCCCTGCGCGCCGTCATCTCCCAGAC
CCTGCTCAAGCGGATCGGCGGGGGTCGGGTGGCGGCCCACGAGATTATGATGGGTATTCCCGCGGTGCGAAACCTCATCC
GGGAAGACAAGATTGCCCAGCTTTATTCTGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGTTGGTCAACCGGGGCATAGTCGCCGCCCTCGACGCCAAGGCCAAGGCGGCCGATCCCAATAGTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

78.134

99.709

0.779
  pilT Acinetobacter nosocomialis M2

76.744

100

0.767
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.453

100

0.765
  pilT Pseudomonas aeruginosa PAK

75.581

100

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.371

97.965

0.738
  pilT Vibrio cholerae strain A1552

75.371

97.965

0.738
  pilT Legionella pneumophila strain Lp02

75.455

95.93

0.724
  pilT Legionella pneumophila strain ERS1305867

75.455

95.93

0.724
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404

Multiple sequence alignment