Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL255_RS02800 Genome accession   NZ_LR134376
Coordinates   591480..592589 (-) Length   369 a.a.
NCBI ID   WP_042652086.1    Uniprot ID   A0AAE9MGI0
Organism   Aeromonas encheleia strain NCTC12917     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 586480..597589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL255_RS02775 (NCTC12917_00563) - 586609..586836 (+) 228 WP_042652082.1 hypothetical protein -
  EL255_RS02780 (NCTC12917_00564) brnQ 586931..588193 (-) 1263 WP_042652199.1 branched-chain amino acid transport system II carrier protein -
  EL255_RS02785 (NCTC12917_00565) - 588430..589140 (-) 711 WP_042652083.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  EL255_RS02790 (NCTC12917_00566) srmB 589269..590489 (+) 1221 WP_042652084.1 ATP-dependent RNA helicase SrmB -
  EL255_RS02795 (NCTC12917_00567) yaaA 590675..591448 (-) 774 WP_042652085.1 peroxide stress protein YaaA -
  EL255_RS02800 (NCTC12917_00568) pilU 591480..592589 (-) 1110 WP_042652086.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL255_RS02805 (NCTC12917_00569) pilT 592615..593649 (-) 1035 WP_042652087.1 type IVa pilus ATPase TapT Machinery gene
  EL255_RS02810 (NCTC12917_00570) - 593689..594390 (+) 702 WP_042652088.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL255_RS02815 (NCTC12917_00571) proC 594474..595298 (+) 825 WP_042652089.1 pyrroline-5-carboxylate reductase -
  EL255_RS02820 (NCTC12917_00572) - 595328..595879 (+) 552 WP_042652090.1 YggT family protein -
  EL255_RS02825 (NCTC12917_00573) yggU 595879..596172 (+) 294 WP_042652091.1 DUF167 family protein YggU -
  EL255_RS02830 (NCTC12917_00574) - 596192..596611 (+) 420 WP_042652092.1 DUF4426 domain-containing protein -
  EL255_RS02835 (NCTC12917_00575) - 596695..597525 (-) 831 WP_042652093.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40868.99 Da        Isoelectric Point: 6.1445

>NTDB_id=1122430 EL255_RS02800 WP_042652086.1 591480..592589(-) (pilU) [Aeromonas encheleia strain NCTC12917]
MNMDVLLAELVAQKGSDLFITVDAPPTLKVNGRLVSLGDKPLDRPAALALVRESLDAEHFERYLHSREANYAIHREALGR
FRVSAFWQQDMPGMVLRRIETHIPSFGELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLITQREVGIDTESFDVALKNSMRQAPDVILIGEIRNQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPEAKHRQFLFDLSFNLRAIVAQQLLPCTDGSRRVAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGSLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1122430 EL255_RS02800 WP_042652086.1 591480..592589(-) (pilU) [Aeromonas encheleia strain NCTC12917]
ATGAACATGGATGTTCTGCTGGCTGAACTGGTTGCCCAAAAGGGCTCGGATCTGTTCATCACGGTCGATGCCCCGCCCAC
CCTCAAGGTCAATGGGCGCCTGGTGTCGCTCGGCGATAAACCGCTGGACAGGCCGGCTGCGCTGGCCCTGGTCAGGGAGA
GCCTGGACGCGGAGCACTTCGAGCGCTACCTGCATAGCCGGGAGGCGAACTACGCCATCCATCGCGAGGCGCTGGGACGG
TTCCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCATATCCCCAGCTTCGG
CGAGCTGGTGCTGCCCCCCATCCTGCAGGAGGTGGCCATGGCCAAGCGCGGGCTGGTGCTGTTTGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATTGGTTATCGCAACCAGCACGGGGACGGTCACATACTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGTCGCTGCCTGATCACCCAGCGGGAGGTCGGCATCGATACCGAATCCTTCGATGT
GGCGCTCAAGAACTCCATGCGCCAGGCGCCGGATGTCATCCTCATCGGCGAGATCCGCAACCAGGAGACCATGGAGCTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGTCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCAGAGGCGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCGCAGCA
ACTGCTGCCCTGCACCGATGGCAGCCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGCGAGATGCATCGGCTCAAGGAGGTGATGACCAAATCCAGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCACGCTGACTCCGCCAACGACCTGCGGCT
GCTGATCAAGCTGTCCGGTCGTGAGCAGCTGGGCGCAGGTTCATTGGACAACGTCACCCTGGACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.967

99.729

0.588
  pilU Pseudomonas stutzeri DSM 10701

58.12

95.122

0.553
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment