Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   EL239_RS05505 Genome accession   NZ_LR134374
Coordinates   1266501..1266911 (-) Length   136 a.a.
NCBI ID   WP_126338720.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1261501..1271911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS05490 (NCTC12082_01116) letS 1261807..1264563 (+) 2757 WP_058482311.1 two-component system sensor histidine kinase LetS Regulator
  EL239_RS05495 (NCTC12082_01117) - 1264593..1265828 (+) 1236 WP_126338718.1 6-phosphofructokinase -
  EL239_RS05500 (NCTC12082_01118) pilA2 1265986..1266393 (-) 408 WP_126338719.1 pilin Machinery gene
  EL239_RS05505 (NCTC12082_01119) pilA2 1266501..1266911 (-) 411 WP_126338720.1 pilin Machinery gene
  EL239_RS05510 (NCTC12082_01120) - 1267264..1267581 (+) 318 WP_126338721.1 BolA family protein -
  EL239_RS05515 (NCTC12082_01121) - 1267574..1268017 (+) 444 WP_126338722.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  EL239_RS05520 (NCTC12082_01122) - 1268091..1269488 (+) 1398 WP_126339973.1 APC family permease -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13971.09 Da        Isoelectric Point: 6.1824

>NTDB_id=1122400 EL239_RS05505 WP_126338720.1 1266501..1266911(-) (pilA2) [Legionella spiritensis strain NCTC12082]
MKQRGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLNLASSAKLAVSETAITNNALPATQAETGYVSPAATPNV
TSIAIGANGVITITYTAAAGGGTLLMTPTLQPNGDVTWDCATGGSLLDKYRPASCR

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1122400 EL239_RS05505 WP_126338720.1 1266501..1266911(-) (pilA2) [Legionella spiritensis strain NCTC12082]
ATGAAACAGAGAGGTTTTACACTGATTGAGTTGATGATTGTGGTCGCCATTGTAGGTATTCTGGCCGCGATTGCAATACC
GGCCTATCAGGATTATACCATTCGCGCCCGGGTTACCGAGGGATTGAACCTGGCGTCATCAGCCAAACTGGCTGTTTCCG
AAACGGCAATCACTAATAATGCCTTACCGGCCACTCAGGCTGAAACTGGCTATGTCAGTCCGGCCGCAACGCCTAATGTG
ACTTCCATAGCAATCGGTGCGAACGGTGTCATCACGATTACCTATACCGCGGCTGCAGGCGGTGGTACCCTTCTCATGAC
TCCAACATTGCAGCCGAACGGTGATGTGACCTGGGATTGTGCTACCGGTGGTTCATTGCTCGATAAATATCGTCCGGCAA
GTTGCAGGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

89.706

100

0.897
  pilA2 Legionella pneumophila strain ERS1305867

89.706

100

0.897
  pilA Ralstonia pseudosolanacearum GMI1000

50.932

100

0.603
  comP Acinetobacter baylyi ADP1

49.315

100

0.529
  pilE Neisseria gonorrhoeae MS11

39.103

100

0.449
  pilE Neisseria gonorrhoeae strain FA1090

37.179

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.606

100

0.419
  pilA/pilA1 Eikenella corrodens VA1

36.538

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.259

99.265

0.39
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.417

100

0.375
  pilA Vibrio cholerae strain A1552

35.417

100

0.375
  pilA Vibrio cholerae C6706

35.417

100

0.375
  pilA Haemophilus influenzae 86-028NP

41.129

91.176

0.375
  pilA Haemophilus influenzae Rd KW20

41.129

91.176

0.375
  pilA Vibrio parahaemolyticus RIMD 2210633

38.462

95.588

0.368
  comF Acinetobacter baylyi ADP1

34.965

100

0.368

Multiple sequence alignment